Browsing by Subject "Blood Coagulation"

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    Conformational activation of antithrombin by heparin involves an altered exosite interaction with protease
    (Elsevier; American Society for Biochemistry and Molecular Biology, 2014-12-05) Águila Martínez, Sonia; Izaguirre, Gonzalo; Qi, Lixin; Swanson, Richard; Roth, Ryan; Rezaie, Alireza R.; Gettins, Peter G. W.; Olson, Steven T.; Medicina Interna
    Background: Exosites are known to mediate heparin allosteric activation of antithrombin. Results: Mutagenesis revealed that an exosite differentially contributes to antithrombin reactivity with factors Xa/IXa in unactivated and heparin-activated states. Conclusion: Heparin allosteric activation of antithrombin results from alterations in an exosite interaction with protease induced by core conformational changes. Significance: The findings support our recently proposed model of antithrombin allosteric activation.