Browsing by Subject "Biomaterial"

Now showing 1 - 2 of 2
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    Biological response to porcine xenograft implants: an experimental study in rabbits
    (Lippincott Williams & Wilkins, 2012-04) Calvo Guirado, José Luis; Gómez Moreno, Gerardo; Guardia, Javier; Ortiz Ruiz, Antonio José; Piatelli, Adriano; Barone, Antonio; Martínez González, José María; Meseguer Olmo, Luis; López Marí, Laura; Barona Dorado, Cristina; Dermatología, Estomatología, Radiología y Medicina Física
    Purpose: The aim of this study was to evaluate the effect of a new porcine biomaterial and collagen paste in 20 New Zealand rabbits. Materials and Methods: Forty implants using a porcine xenograft made up of 80% corticocancellous collagenated bone particles of ≤300 μm in size were placed in the proximal metaphyseal area of both tibiae. Four periods of time were formed: 1h, 5, 8, and 15 months. After implantation, an anteroposterior and lateral radiological study was carried out. Samples were sectioned at 5 μm and stained using hematoxylin-eosin, Masson's trichromic, and Gordon-Switt reticulin stains. Results: These results confirmed the biocompatibility of this porcine biomaterial-collagen paste; only a few, occasional macrophages and scattered lymphocytes were observed. No fibrosis was observed between the implants and the bone. Moreover, the material was osteoconductive acting as a “scaffold” for bone cells, and there was a progressive increase in bone growth in and around the implants. Conclusion: This new porcine biomaterial-collagen paste seemed to be biocompatible, bioresorbable, and osteoconductive.
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    Histopathological and statistical study of corneal repair in rabbits. A new way to understand corneal recovery
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2024) Ramírez Perdomo, Patricia; Hernández Marrero, Juan Daniel; Guillén Molina, Santiago; Saavedra Santana, Pedro; León Vera, Mónica de; Araujo Ruano, Eduardo José; Loro Ferrer, Juan Francisco; Cabrera Galván, Juan José; Ramírez González, Juan Andrés
    The study aims to evaluate corneal healing post amniotic membrane transplantation in controlled corneal defects, justifying its application in routine ophthalmology practice. The objective is to establish a reliable method for assessing the repair process. In three groups of six adult New Zealand rabbits, keratectomy and a monolayer transplant of dehydrated human amniotic membrane (AM) were conducted in the left eye (OS) with the right eye (OD) serving as the control eye. Clinical signs were assessed, and both eyes were enucleated at 1, 2, and 4 weeks for optical coherence tomography (OCT) measurements and histological analysis, collecting data from different epithelium, stroma, and limbus regions. This study was conducted using a formula that combines histologic data categorizing their presence and/or type as beneficial for corneal repair. No statistically significant differences were found between the experimental and control eyes regarding all clinical signs and OCT measurements. However, a linear model using histopathological results showed a period-implant mode interaction with statistical significance (P=0.010). The use of the single-layer amniotic membrane resulted in improved corneal recovery with the stromal side showing better performance in the first week and the epithelial side proving to be more effective than the stromal side in the long term. For the first time, a statistical formula employing histopathological data is introduced to determine corneal recovery, potentially offering a more accurate and reliable method compared with the observation of clinical signs and corneal measurements with OCT.