Browsing by Subject "Biological activity"

Now showing 1 - 2 of 2
  • Thumbnail Image
    Publication
    Restricted
    Inactivation of lipoxygenase and cyclooxygenase by natural betalains and semi-synthetic analogues
    (Elsevier, 2014-01-13) Vidal, Pedro J.; Gandía Herrero, Fernando; López Nicolás, José Manuel; García Carmona, Francisco; Bioquímica y Biología Molecular "A"
    Betalains are natural pigments characteristic of plants of the order Caryophyllales. In this work, the role of betalains in the anti-inflammatory activity described for plant extracts is analysed in terms of the inactivation of the enzymes involved in the biochemical response (lipoxygenase and cyclooxygenase). Pure natural betalains and semi-synthetic analogues are demonstrated to promote a significant reduction of the enzymes activity. Reactions were followed spectrophotometrically and by HPLC-DAD. Phenethylamine-betaxanthin was the most potent in the inactivation of cyclooxygenase, with a reduction of 32% of the control activity at 125 lM, while the natural pigment betanidin and a betalain analogue derived from indoline resulted as the most potent inactivators of lipoxygenase, with IC50 values of 41.4 and 40.1 lM, respectively. Molecular docking studies revealed that betalains interact with the lipoxygenase amino acids involved in substrate binding and with Tyr-385 and Ser-530 close to the yclooxygenase active site, interfering in enzyme catalysis.
  • Thumbnail Image
    Publication
    Open Access
    Recovery of pure C-phycoerythrin from a limestone drought tolerant cyanobacterium Nostoc sp. and evaluation of its biological activity
    (Universidad de Murcia. Servicio de publicaciones, 2020) Nowruzi, Bahareh; Fahimi, Hossein; Sturion Lorenzi, Adriana
    Se realizó la caracterización de ficoeritrina de la cepa A5 de Nostoc sp., seguida de investigación de su actividad biológica para aplicaciones biotecnológicas. Para la extracción de ficoeritrina, el uso de tampón acetato (pH 5.1) produjo 65.04 µg mL-1, y se identificó como C-ficoeritrina. Los resultados de su actividad antioxidante sugirieron su acción como un potente eliminador de radicales libres. Además, la C-ficoeritrina de Nostoc mostró una capacidad notable como agente antibacteriano y antifúngico, con estabilidad significativa de hasta 10 días. La glucosa (4 mg mL-1) fue un buen conservante para la C-ficoeritrina a 25 y 4 ºC. Se obtuvo por primera vez una C-ficoeritrina estable de Nostoc sp. en condiciones de sequía en piedra caliza, lo que demuestra la necesidad de estudiar microorganismos de ambientes extremos.