Publication: Recovery of pure C-phycoerythrin from a limestone drought tolerant cyanobacterium Nostoc sp. and evaluation of its biological activity
Authors
Nowruzi, Bahareh ; Fahimi, Hossein ; Sturion Lorenzi, Adriana
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Publisher
Universidad de Murcia. Servicio de publicaciones
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DOI
doi.org/10.6018/analesbio.42.13
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info:eu-repo/semantics/article
Description
Abstract
Se realizó la caracterización de ficoeritrina de la cepa A5 de Nostoc sp., seguida de investigación de su actividad biológica para
aplicaciones biotecnológicas. Para la extracción de ficoeritrina, el
uso de tampón acetato (pH 5.1) produjo 65.04 µg mL-1, y se identificó como C-ficoeritrina. Los resultados de su actividad antioxidante sugirieron su acción como un potente eliminador de radicales libres. Además, la C-ficoeritrina de Nostoc mostró una capacidad
notable como agente antibacteriano y antifúngico, con estabilidad
significativa de hasta 10 días. La glucosa (4 mg mL-1) fue un buen
conservante para la C-ficoeritrina a 25 y 4 ºC. Se obtuvo por primera vez una C-ficoeritrina estable de Nostoc sp. en condiciones de
sequía en piedra caliza, lo que demuestra la necesidad de estudiar
microorganismos de ambientes extremos.
The phycoerythrin characterization from Nostoc sp. strain A5 was done, followed by investigation of its biological activity for biotechnological applications. For phycoerythrin extraction, the use of acetate buffer (pH 5.1) resulted in 65.04 µg mL-1, and C-phycoerythrin was identified. Results of its antioxidant activity suggested action as a potent free radical scavenger. In addition, Nostoc's Cphycoerythrin showed noteworthy ability for antibacterial and antifungal agents with significant stability up to 10 days. Glucose (4 mg mL-1) was a good preservative for C-phycoerythrin at 25 and 4 ºC. A stable C-phycoerythrin from Nostoc sp. was obtained for the first time from limestone drought conditions, showing the need of studying microorganisms from extreme environments.
The phycoerythrin characterization from Nostoc sp. strain A5 was done, followed by investigation of its biological activity for biotechnological applications. For phycoerythrin extraction, the use of acetate buffer (pH 5.1) resulted in 65.04 µg mL-1, and C-phycoerythrin was identified. Results of its antioxidant activity suggested action as a potent free radical scavenger. In addition, Nostoc's Cphycoerythrin showed noteworthy ability for antibacterial and antifungal agents with significant stability up to 10 days. Glucose (4 mg mL-1) was a good preservative for C-phycoerythrin at 25 and 4 ºC. A stable C-phycoerythrin from Nostoc sp. was obtained for the first time from limestone drought conditions, showing the need of studying microorganisms from extreme environments.
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Citation
Anales de Biología, vol.42, (2020)
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