Browsing by Subject "Biocompatibility"

Now showing 1 - 7 of 7
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    Aggregation behaviour of gold nanoparticles in presence of chitosan
    (Springer, 2015-06-13) Fernández Espín, Vanesa ; Pamies, Ramón ; García de la Torre, José; García Montalbán, Mercedes; Collado-González, Mar; Hernández Cifre, José Ginés; Díaz Baños, F. Guillermo; Víllora Cano, Gloria; Ingeniería Química
    Chitosan (CS) is a biocompatible polysaccharide with positive charge that is widely used as a coating agent for negatively charged nanoparticles. However, the types of structures that emerge by combining CS and anoparticles as well as their behaviour are still poorly understood. In this work, we characterize the nanocomposites formed by gold nanoparticles (AuNPs) and CS and study the influence of CS in the expected aggregation process that should experience those nanoparticles under the favourable conditions of low pH and high ionic strength. Thus, at the working CS concentration, we observe the existence of CS structures that quickly trap the AuNPs and avoid the formation of nanoparticle aggregates in environmental conditions that, otherwise, would lead to such an aggregation.
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    Are denture adhesives safe for oral cells?
    (Wiley, 2020-07-12) López García, Sergio; Pecci Lloret, María Pilar; Guerrero Gironés, Julia; Pecci Lloret, Miguel Ramón; Rodríguez Lozano, Francisco Javier; García Bernal, David; Dermatología, Estomatología, Radiología y Medicina Física
    Purpose: To compare the cytotoxicity of six commercially available denture adhesives on human gingival cells: Poligrip Flavour Free Fixative Cream, Fixodent Pro Duo Protection, Novafix cream, FittyDent, Polident Total Action, and Fixodent ProPlus Duo Protection. Material and Methods: Eluates of denture adhesives were brought into contact with human gingival cells and compared to untreated cells (w/o any dental adhesive elute). Cell toxicity was assessed by measuring cell viability (3-(4,5-imethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assays), cell morphology (immunofluorescenceassays), induction of apoptosis/necrosis and production of reactive oxygen species (ROS) (flow cytometry assays). In addition, the pH of each sample was determined. Data were analyzed using one-way analysis of variance (ANOVA) followed by Dunnett’s multiple comparisons test. Results: All denture adhesives tested led to a reduction in pH, especially Fixodent Pro Duo Protection and Fixodent Pro Plus Duo Protection. The cell viability assays showed that Fixodent Pro Duo Protection (1:1 72 hours, p = 3.04 × 10−6; 1:2 72 hours, p = 2.07 × 10−6; 1:4 72 hours, p = 2.04 × 10−6) and Fixodent Pro Plus Duo Protection (1:1 72 hours, p = 2.01 × 10−6; 1:2 72 hours, p = 3.03 × 10−6; 1:4 72 hours, p = 2.02 × 10−6) significantly decreased cell viability at all dilutions. Compared to the control group and the rest of the adhesives, Poligrip Flavour Free Fixative Cream (PFF 1:1 72 hours, p = 2.24 × 10−6; 1:2 72 hours, p = 2.44 × 10−6; 1:4 72 hours, p = 2.04 × 10−6) showed a significantly higher cell viability score at all dilutions. Fixodent Pro Duo Protection and Fixodent Pro Plus Duo Protection, both adhesives containing zinc salts in their composition, were responsible for necrosis, and the number of cells was much reduced, with aberrant morphology and pyknotic nucleus. Finally, Fixodent (1:2, p = 2.04 × 10−6, 1:4, p = 0.00036; 1:2, p = 8.82 × 10−6, 1:4, p = 2.30 × 10−6) products significantly promoted ROS production in gingival cells. Conclusions: The results suggest that denture adhesives containing zinc in their composition could be responsible of the decrease of cell viability, ROS production, aberrant cell morphology, and induction of apoptosis and cell death. However, other possible additional cytotoxic factors must be considered. Thus, more studies are necessary to confirm this hypothesis.
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    Open Access
    Bioceramic versus traditional biomaterials for endodontic sealers according to the ideal properties
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2024) Ortega, Miguel A.; Rios, Laura; Fraile Martínez, Oscar; Liviu Boaru, Diego; Leon Oliva, Diego De; Barrena Blázquez, Silvestra; Pereda Cerquella, Claude; Garrido Gil, Maria J.; Manteca, Luis; Buján, Julia; García Honduvilla, Natalio; Garcia Montero, Cielo; Rios Parra, Antonio
    Odontology, as a scientific discipline, continuously collaborates with biomaterials engineering to enhance treatment characteristics and patients' satisfaction. Endodontics, a specialized field of dentistry, focuses on the study, diagnosis, prevention, and treatment of dental disorders affecting the dental pulp, root, and surrounding tissues. A critical aspect of endodontic treatment involves the careful selection of an appropriate endodontic sealer for clinical use, as it significantly influences treatment outcomes. Traditional sealers, such as zinc oxide-eugenol, fatty acid, salicylate, epoxy resin, silicone, and methacrylate resin systems, have been extensively used for decades. However, advancements in endodontics have given rise to bioceramic-based sealers, offering improved properties and addressing new challenges in endodontic therapy. In this review, a classification of these materials and their ideal properties are presented to provide evidence-based guidance to clinicians. Physicochemical properties, including sealing ability, stability over time and space, as well as biological properties such as biocompatibility and antibacterial characteristics, along with costeffectiveness, are essential factors influencing clinicians' decisions based on individual patient evaluations.
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    Biocompati bility and osteoconductivity of the pyrost bone substitutes
    (Murcia : F. Hernández, 1997) Tsuang, Y.H.; Lin, F.H.; Tai, H.C.; Sun, J.S.; Liu, H.C.; Hang, Y. S.
    The purpose of this study was to re-evaluate the bone regeneration power and the in vitro biocompatibility of the Pyrost bone substitute. Twentyfour adult New Zealand White rabbits were used. Bony defect over both iliac crest and mid-diaphyseal portion of the ulna bone were created. Appropriate sized-block of Pyrost bone substitute were implanted. Four of the animals were killed at each postoperative month to evaluate its bone regeneration power by histologic study. The Pyrost bones were co-cultured with osteoblasts to evaluate its biocompatibility. The results showed that Pyrost bone substitute was quite stable and incorporated well with active bone regeneration. The Pyrost heal better at the iliac crest than at the ulnar defect. The Pyrost was compatible to the osteoblasts. Osteoblasts had successfully seeded and mitotically expanded on the porous surface of the Pyrost bone graft. The result showed that Pyrost bone obviously exerts an intense stimulus on osteo-regeneration in the presence of osteoblasts. We consider Pyrost to be an altemate to the conventional preserved allografts that is occasionally necessary.
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    Biocompatibility Evaluation of Four Dentin Adhesives Used as Indirect Pulp Capping Materials
    (2017) Cortés, Olga; Alcaina, Antonia; Bernabe, Antonia; Dermatología, Estomatología, Radiología y Medicina Física
    In many cases, the indirect pulp treatment (IPT) is an acceptable treatment for deciduous teeth with reversible pulp inflammation. Various medicaments have been used for IPT, ranging from calcium hy- droxide and glass ionomers to dentin adhesives. Objective: This in vitro trial aimed to measure cyto- toxicity in a cell culture, comparing the following four adhesives: Xeno® V (XE), Excite® F DSC (EX), Adhese® OneF (AD) and Prime & Bond NT (PB). Materials and methods: The adhesives were pre- pared according to the manufacturer’s instructions. After 24 hours of exposure, the cell viability was evaluated using a photometrical test (MTT test). Data were subjected to analysis of variance (ANO- VA). Results: Adhesives, the main component of which was 2-hydroxyethyl methacrylate (HEMA), were found to be less cytotoxic, while those that included the monomer urethane dimethacrylate (UDMA were the most cytotoxic ) in their composition. The effects on cell viability assay varied be- tween the adhesives assayed with statistically significant differences. Conclusions: The results may support the argument that Adhese® OneF is the least cytotoxic of the adhesives assayed, and may be considered as an adhesive agent for indirect pulp treatment. However, Prime and Bond NT showed a reduced biocompatibility under the same conditions.
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    Biological properties of Ceraputty as a retrograde filling material: an in vitro study on hPDLSCs
    (Springer, 2023-05-01) López Garcia, Sergio; Rodríguez Lozano, Francisco Javier; Sanz, José Luis; Forner, Leopoldo; Pecci Lloret, María Pilar; Lozano, Adrián; Murcia, Laura; Sánchez Bautista, Sonia; Oñate Sánchez, Ricardo Elías; Dermatología, Estomatología, Radiología y Medicina Física
    Objectives To assess the cytocompatibility and bioactive potential of the new calcium silicate-based cement Ceraputty on human periodontal ligament stem cells (hPDLSCs) compared to Biodentine and Endosequence BC root repair material (ERRM). Materials and methods hPDLSCs were isolated from extracted third molars from healthy donors. Standardized sample discs and 1:1, 1:2, and 1:4 eluates of the tested materials were prepared. The following assays were performed: surface element distribution via SEM–EDX, cell attachment and morphology via SEM, cell viability via a MTT assay, osteo/cemento/odontogenic marker expression via RT-qPCR, and cell calcified nodule formation via Alizarin Red S staining. hPDLSCs cultured in unconditioned or osteogenic media were used as negative and positive control groups, respectively. Statistical analysis was performed using one-way ANOVA or two-way ANOVA and Tukey’s post hoc test. Statistical significance was established at p < 0.05. Results The highest Ca2+peak was detected from Biodentine samples, followed by ERRM and Ceraputty. hPDLSC viability was significantly reduced in Ceraputty samples (p < 0.001), while 1:2 and 1:4 Biodentine and ERRM samples similar results to that of the negative control (p > 0.05). Biodentine and ERRM exhibited an upregulation of at least one cemento/odonto/osteogenic marker compared to the negative and positive control groups. Cells cultured with Biodentine produced a significantly higher calcified nodule formation than ERRM and Ceraputty (p < 0.001), which were also higher than the control groups (p < 0.001). Conclusion Ceraputty evidenced a reduced cytocompatibility towards hPDLSCs on its lowest dilutions compared to the other tested cements and the control group. Biodentine and ERRM promoted a significantly higher mineralization and osteo/cementogenic marker expression on hPDLSCs compared with Ceraputty. Further studies are necessary to verify the biological properties of this new material and its adequacy as a retrograde filling material.
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    Estudio de la biocompatibilidad intraósea de dos materiales dentales de alto contenido en calcio
    (Ergon Ediciones S. L., 2002) Chiva García, Fernando; Carrascosa García, J.; López Jornet, Pía; Ortiz Ruiz, Antonio José; Vicente Ortega, Vicente; Dermatología, Estomatología, Radiología y Medicina Física