Browsing by Subject "Basal cell"

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    A clinicopathological study of eight cases presenting a biphasic structure: A distinct variant of pulmonary carcinoma
    (2026) Qiuyao Li; Jiwei Ma; Kun Yang; Xiaoyan Lin; Huifeng Jiang; Yali Xu; Lin Song; Yu Zhang; Xiaoqian Liu; Zheng Mou; Wenjing Su; Hongyu Wang; Biología Celular e Histología; Universidad de Murcia, Departamento de Biologia Celular e Histiologia
    Biphasic structures, which are composed of outer basal cells and inner glandular cells, are frequently indicative of salivary gland-type tumors or benign lesions, such as bronchial adenoma, within the lung. However, the occurrence of a biphasic structure in lung cancer is rarely reported and can lead to significant challenges and confusion in diagnosis, particularly in biopsy specimens. In our study, we collected eight lung epithelial tumors that presented with a distinct biphasic structure component and examined their clinicopathological characteristics. Histological examination revealed that the biphasic structure component, often intermingled with conventional squamous cell carcinoma or adeno carcinoma, was defined by basal cells encircling the glandular epithelium. Immunohistochemical analysis demonstrated a distinctive peripheral p40 staining pattern in the biphasic structure component. Genetic analysis identified driver mutations in seven out of eight patients, which are typically associated with conventional pulmonary adenocarcinomas, including EGFR L858R, EGFR 19-del, EGFR 20-ins, and KRAS mutations. The presence of biphasic structure components in these cases confirms a genuine form of lung cancer, likely representing a variant of lung adenocarcinoma. This study's findings enhance the understanding of lung cancer's morphological diversity and caution against prematurely dismissing the malignancy potential of pulmonary epithelial lesions based solely on the presence of basal cells, especially with biopsy specimens.
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    T-2 toxin-induced acute skin lesions in 3 Wistar-derived hypotrichotic WBNIILA-Ht rats
    (Murcia : F. Hernández, 1999) Albarenque, S.M.; Shinozuka, J.; Iwamoto, S.; Nakayama, Hiroyuki; Doi, K.
    Acute lesions in the dorsal skin topically applied with T-2 toxin (10 p1 of 0.5 mg/ml-solution to lcm2) were examined in Wistar-derived hypotrichotic WBNIILA-Ht rats up to 24 hours after treatment (24HAT). In the epidermis, depression of basal cell proliferating activity was detected at 3HAT by immunostaining for proliferating cell nuclear antigen (PCNA), and the percentage of PCNA-positive basal cells decreased thereafter. At 12HAT, in addition to intracytoplasmic edema of spinous cells, acidophilic degeneration of basal cells characterized by shrinkage of cell body with acidophilic cytoplasm and pyknotic or karyorrhectic nuclei became prominent. Most of these nuclei were positive for TUNEL which is a widely used immunostaining for the in situ detection of fragmented DNA, i.e. apoptosis, and the percentage of TUNELpositive basal cells increased thereafter. The nuclei of these basal cells also showed ultrastructural changes characteristic for apoptosis. On the other hand, in the dermis, infiltration of inflammatory cells including mast cells started at 3HAT and increased thereafter. In addition, capillary and small vessel endothelial degeneration developed at 6HAT and progressed thereafter. These results suggest that T-2 toxin directly affects the epidermis and produces apoptosis in basal cells.