Publication: T-2 toxin-induced acute skin lesions in 3 Wistar-derived hypotrichotic WBNIILA-Ht rats
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Date
1999
Authors
Albarenque, S.M. ; Shinozuka, J. ; Iwamoto, S. ; Nakayama, Hiroyuki ; Doi, K.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Acute lesions in the dorsal skin topically
applied with T-2 toxin (10 p1 of 0.5 mg/ml-solution to
lcm2) were examined in Wistar-derived hypotrichotic
WBNIILA-Ht rats up to 24 hours after treatment
(24HAT). In the epidermis, depression of basal cell
proliferating activity was detected at 3HAT by immunostaining
for proliferating cell nuclear antigen (PCNA),
and the percentage of PCNA-positive basal cells
decreased thereafter. At 12HAT, in addition to intracytoplasmic
edema of spinous cells, acidophilic degeneration
of basal cells characterized by shrinkage of cell body
with acidophilic cytoplasm and pyknotic or karyorrhectic
nuclei became prominent. Most of these nuclei
were positive for TUNEL which is a widely used
immunostaining for the in situ detection of fragmented
DNA, i.e. apoptosis, and the percentage of TUNELpositive
basal cells increased thereafter. The nuclei of
these basal cells also showed ultrastructural changes
characteristic for apoptosis. On the other hand, in the
dermis, infiltration of inflammatory cells including mast
cells started at 3HAT and increased thereafter. In
addition, capillary and small vessel endothelial
degeneration developed at 6HAT and progressed
thereafter. These results suggest that T-2 toxin directly
affects the epidermis and produces apoptosis in basal
cells.
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