Browsing by Subject "Assay validation"

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    Data for compounds included in the article: "A sensitive immunoassay for the quantitation of Pig-MAP in pig saliva samples"
    (2024-10-05) Piñeiro, M; Matas-Quintanilla, M; Miralles, A; Gutiérrez, AM; Medicina y Cirugía Animal
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    New potential biomarkers of oxidative stress in Mytilus galloprovincialis: Analytical validation and overlap performance
    (Elsevier, 2018-04-23) Franco-Martínez, Lorena; Romero, Diego; Peres Rubio, Camila; Tecles, Fernando; Martínez-Subiela, Silvia; Teles, Mariana; Tvarijonaviciute, Asta; Ciencias Sociosanitarias
    The aim of the present report was to develop and validate new automated spectrophotometric assays for measurement of total antioxidant capacity (TAC), thiols, and advanced oxidation protein products (AOPP) in mussel gills, digestive gland and hemolymph samples, and to evaluate their possible utility in biomonitoring programs. Total antioxidant capacity (TAC) was measured by different methods: trolox equivalent antioxidant capacity (TEAC1 and TEAC2), cupric reducing antioxidant capacity (CUPRAC), and ferric reducing ability of plasma (FRAP). The assays were precise, accurate and provided low limits of detection. When oxidative stress was promoted by inducing hypoxia and the behaviour of these biomarkers between hypoxic and controls mussels were compared, statistically significant differences were observed in all biomarkers and tissues evaluated. The results of the present study demonstrated that these biomarkers, not previously studied in mussels, show a potential use as biomarkers of oxidative stress in this species since they were validated and showed changes under a state of oxidative stress.
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    Serum paraoxonase type-1 activity in pigs: assay validation and evolution after an induced experimental inflammation
    (Elsevier, 2015-02-15) Escribano Tortosa, Damián; Tvarijonaviciute, Asta; Tecles Vicente, Fernando; Cerón Madrigal, José J.; Producción Animal
    Paraoxonase 1 (PON1) is a serum enzyme synthesised and secreted primarily by the liver. It possesses anti-inflammatory properties limiting the production of pro-inflammatory mediators. The objectives of this study were to validate three spectrophotometric assays for the quantification of PON1 activity in pig serum, and to determine if PON1 activity in porcine behaves as a negative acute phase protein (APP), decreasing in inflammatory conditions. An analytical validation using three different substrates – 5-thiobutil butyrolactone (TBBL), phenylacetate (PA) and 4-(p)-nitrophenyl acetate (pNA) – was performed. In addition, inflammation was experimentally induced in five pigs by subcutaneous injection of turpentine oil, while five control pigs were left untreated. The treated pigs showed significant increases in CRP and decreases in albumin, indicating an inflammatory condition. The three substrates used would be suitable for PON1 activity measurements in serum samples, since they offer adequate precision (coefficients of variation < 10%), sensitivity (0.01, 0.15, 0.02 U/mL for TBBL, pNA and PA respectively) and accuracy (r = 0.99). In addition, PON1 behaves as a negative APP in pigs since a significant decrease (P < 0.05) in its activity after 72 h of the induction of the inflammation was observed with all substrates.