Browsing by Subject "Alpha amylase"

Now showing 1 - 4 of 4
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    Autonomic markers associated with generalized social phobia symptoms: heart rate variability and salivary alpha-amylase
    (Taylor and Francis Group, 2017) Espín López, Laura; García Rubio, María J.; Hidalgo, Vanesa; Salvador, Alicia; Gómez Amor, Jesús; Anatomía Humana y Psicobiología; Facultades de la UMU::Facultad de Psicología y Logopedia
    The study of autonomic nervous system changes associated with generalized social phobia (GSP) dis- order has increased in recent years, showing contradictory results. The present study aimed to evaluate how young people with GSP reacted before, during, and after exposure to the Trier Stress Social Test (TSST), focusing on their autonomic changes (heart rate variability (HRV) and salivary alpha-amylase (sAA)) compared to a control group (non-GSP). Some psychological variables were also considered. Sex was specifically studied as a possible modulator of autonomic fluctuations and psychological state. Eighty young people were randomly distributed into two counterbalanced situations: stress condition (N1⁄418 and 21 for GSP and non-GSP, respectively) and control condition (N1⁄421 and 20 for GSP and non-GSP, respectively), where cardiovascular variables were continuously recorded. Psychological ques- tionnaires about mood and perceived stress were filled out, and five saliva samples were collected to analyze sAA. GSP participants showed higher values on low- and high-frequency ratios (HR domains), compared to non-GSP people, during exposure to the TSST, but no differences were observed after the stressor. Furthermore, the two groups did not differ in sAA. Importantly, positive affect in GSP partici- pants was modulated by sex. The present study suggests that the balance between high- and low-fre- quency domains of HRV is a key cardiovascular marker reflecting the stress response of GSP people, as well the importance of sex in positive affect when facing a stressful situation.
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    Enhancing effects of acute psychosocial stress on priming of non-declarative memory in healthy young adults
    (Taylor and Francis Group, 2012) Espín López, Laura; Hidalgo, Vanesa; Villada, Carolina; Almela, Mercedes; Gómez Amor, Jesús; Salvador, Alicia; Anatomía Humana y Psicobiología; Facultades de la UMU::Facultad de Psicología y Logopedia
    Social stress affects cognitive processes in general, and memory performance in particular. However, the direction of these effects has not been clearly established, as it depends on several factors. Our aim was to determine the impact of the hypothalamus–pituitary–adrenal (HPA) axis and sympathetic nervous system (SNS) reactivity to psychosocial stress on short-term non-declarative memory and declarative memory performance. Fifty-two young participants (18 men, 34 women) were subjected to the Trier Social Stress Task (TSST) and a control condition in a crossover design. Implicit memory was assessed by a priming test, and explicit memory was assessed by the Rey Auditory Verbal Learning Test (RAVLT). The TSST provoked greater salivary cortisol and salivary alpha-amylase (sAA) responses than the control task. Men had a higher cortisol response to stress than women, but no sex differences were found for sAA release. Stress was associated with an enhancement of priming but did not affect declarative memory. Additionally, the enhancement on the priming test was higher in those whose sAA levels increased more in response to stress (r48 1⁄4 0.339, p 1⁄4 0.018). Our results confirm an effect of acute stress on priming, and that this effect is related to SNS activity. In addition, they suggest a different relationship between stress biomarkers and the different memory systems.
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    Evaluation of the effect of a live interview in journalism students on salivary stress biomarkers and conventional stress scales
    (Multidisciplinary Digital Publishing Institute, 2022-02-09) Roca Marín, Delfina; Escribano Tortosa, Damián; Franco Martínez, Lorena; Contreras Aguilar, María Dolores; Bernal Gambín, Luis Jesús; Cerón Madrigal, José Joaquín; Rojo Villada, Pedro Antonio; Martínez Subiela, Silvia; Tvarijonaviciute, Asta; Comunicación; Facultades de la UMU::Comunicación y Documentación
    A career in journalism can be very stressful, as journalists frequently have to deal with uncontrolled situations such as conducting live interviews. Therefore, training is essential during their career, both for the development of communication skills and for the improvement of the real and effective capacity to perform the tasks of their professional activity. The aim of this study was to assess the levels of stress in students before and after a practical training in a professional television set using subjective (State-Trait Anxiety Inventory (STAI) and Likert scale) and objective (salivary cortisol and alpha-amylase) methods. The results indicate that a live interview produces stress in the students as revealed by increased concentrations of cortisol and alpha amylase in saliva. Furthermore, students with lower initial concentrations of these biomarkers obtained better grades in evaluation, suggesting that greater control of anticipatory stress could be associated with a better activity performance.
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    Salivary D-dimer in pigs: Validation of an automated assay and changes after acute stress
    (Elsevier, 2020-05-31) López Martínez, M. J.; Escribano, D.; Contreras Aguilar, M. D.; García Martínez, Juan Diego; Martínez Subiela, S.; Cerón, J. J.; Medicina y Cirugía Animal
    D-dimer is a peptide found in serum and is derived from the degradation of blood clots. Even though it has been analysed in human saliva, D-dimer has not been previously evaluated in the saliva of any veterinary species, and its source and role remain unknown. The objectives of this research were firstly, to validate the use of an automated method for the measurement of D-dimer in porcine saliva, and secondly, to evaluate whether D-dimer concentration changes in pig saliva after an acute stress stimulus. For this purpose, a complete analytical validation of a commercially-available immunoturbidimetric assay was carried out. In addition, an experimental acute stress model was induced in 11 pigs based on a technique involving restraint by nose-snare immobilisation for 1 min. Saliva samples were subsequently collected at different times and D-dimer, salivary alpha-amylase (sAA) and cortisol were assessed in order to evaluate changes in its concentrations after the stress induction. The D-dimer automated assay showed adequate reproducibility and sensitivity, with coefficients of variation below 10% and a limit of quantification of 0.167 μg/mL fibrinogen equivalent units (FEU). It also showed a high accuracy, determined by linearity under dilution and recovery tests. In the stress model, a significant increase (P < 0.05) in salivary D-dimer 15 min after the stress stimulus and a positive correlation between D-dimer and sAA (r = 0.51; P < 0.001) were observed. These results indicate that D-dimer can be measured in porcine saliva with an automated method and suggest that its concentration can be influenced by stressful conditions.