Browsing by Subject "Adhesion"
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- PublicationOpen AccessBiocompatibility of new pulp-capping materials NeoMTA Plus, MTA Repair HP and Biodentine on human dental pulp stem cells(Elsevier Science, 2017-11-01) Tomás Catalá, Christopher Joseph; Collado-González, Mar; García Bernal, David; Oñate Sánchez, Ricardo Elías; Forner, Leopoldo; Llena, Carmen; Lozano, Adrián; Moraleda Jiménez, José María; Rodríguez Lozano, Francisco Javier; Biología Celular e HistologíaIntroduction: The aim of the present study was to evaluate the in vitro cytotoxicity of MTA Repair HP, Neo-MTA Plus, and Biodentine, new bioactive materials used for dental pulp capping, on human dental pulp stem cells (hDPSCs). Methods: Biological testing was carried out in vitro on hDPSCs. Cell viability and cell migration assays were performed using eluates of each capping material. To evaluate cell morphology and cell attachment to the different materials, hDPSCs were directly seeded onto the material surfaces and analyzed by scanning electron microscopy. The chemical composition of the pulp-capping materials was determined by energy-dispersive X-ray and eluates were analyzed by inductively coupled plasma-mass spectrometry. Statistical differences were assessed by analysis of variance and Tukey test (P < .05). Results: Cell viability was moderate after 24 and 48 hours in the presence of MTA Repair HP and NeoMTA Plus, whereas at 48 and 72 hours, Biodentine showed higher rates of cell viability than MTA Repair HP and NeoMTA Plus (P < .001). A cell migration assay revealed adequate cell migration rates for MTA Repair HP and NeoMTA Plus, both similar to the control group rates, meanwhile the highest cell migration rate was observed in the presence of Biodentine (P < .001). Scanning electron microscope studies showed a high degree of cell proliferation and adhesion on Biodentine disks but moderate rates on MTA Repair HP and NeoMTA Plus disks. Energydispersive X-ray pointed to similar weight percentages of C, O, and Ca in all 3 materials, whereas other elements such as Al, Si, and S were also found. Conclusions: The new pulp-capping materials MTA Repair HP, NeoMTA Plus, and Biodentine showed a suitable degree of cytocompatibility with hDPSCs, and good cell migration rates, although Biodentine showed higher rates of proliferation time-dependent.
- PublicationOpen AccessCell adhesion molecules in human osteoblasts: structure and function(Murcia : F. Hernández, 2001) Bennett, J.H.; Moffatt, S.; Horton, M.Osteoblasts and bone lining cells form a near continuous layer covering the bone surface and interactions between these cells and the organic matrix of bone are important determinants of osteoblast proliferation and differentiation. In addition, cells of the osteoblast-lineage form functional cornrnunications with each other, with the extra-cellular matrix and with osteocytes through cytoplasmic processes extending through canaliculi in the bone. Together, these cells form a network of putative importance in the regulation of skeletal homeostasis. Cell-cell and cell-matrix interactions are mediated by members of severa1 families of cell adhesion molecules, and knowledge of their interactions will be of fundamental importance in understanding the role of osteoblast in skeletal turnover . Here, the expression pattern of members of the major families of cell adhesion molecules by cells of the osteoblast lineage is reviewed. Special emphasis has been placed on human tissues. In addition, the possibility that cells at progressive stages of the osteoblast lineage have different profiles of cell adhesion molecule expression is explored, and the putative significance of cell-matrix interactions in human skeletal disease briefly discussed.
- PublicationOpen AccessHow galectins have become multifunctional proteins(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2020) García Caballero, Gabriel; Kaltner, Herbert; Kutzner, Tanja J.; Ludwig, Anna-Kristin; Manning, Joachim C.; Schmidt, Sebastian; Sinowatz, Fred; Gabius, Hans-JoachimHaving identified glycans of cellular glycoconjugates as versatile molecular messages, their recognition by sugar receptors (lectins) is a fundamental mechanism within the flow of biological information. This type of molecular interplay is increasingly revealed to be involved in a wide range of (patho)physiological processes. To do so, it is a vital prerequisite that a lectin (and its expression) can develop more than a single skill, that is the general ability to bind glycans. By studying the example of vertebrate galectins as a model, a total of five relevant characteristics is disclosed: i) access to intra- and extracellular sites, ii) fine-tuned gene regulation (with evidence for co-regulation of counterreceptors) including the existence of variants due to alternative splicing or single nucleotide polymorphisms, iii) specificity to distinct glycans from the glycome with different molecular meaning, iv) binding capacity also to peptide motifs at different sites on the protein and v) diversity of modular architecture. They combine to endow these lectins with the capacity to serve as multi-purpose tools. Underscoring the arising broad-scale significance of tissue lectins, their numbers in terms of known families and group members have steadily grown by respective research that therefore unveiled a well-stocked toolbox. The generation of a network of (ga)lectins by evolutionary diversification affords the opportunity for additive/synergistic or antagonistic interplay in situ, an emerging aspect of (ga)lectin functionality. It warrants close scrutiny. The realization of the enormous potential of combinatorial permutations using the five listed features gives further efforts to understand the rules of functional glycomics/lectinomics a clear direction.
- PublicationOpen AccessPhysicochemical characteristics of fibre sources and their effects on in vitro Escherichia coli infection in IPEC-J2 cells(Taylor & Francis, 2025-05-09) Ruiz, Eduardo; Martínez Miró, Silvia; Hernández Ruipérez, Fuensanta; Ayala García, Lucía; Madrid Sánchez, Josefa; Ortega Hernández, Nieves; Producción AnimalFeed-related strategies, such as adding fibre to weaned-piglets’ feed, have been suggested to reduce the impact of Escherichia coli (E. coli) in swine production. We performed physicochemical analysis of fibre sources (wheat bran, commercial insoluble fibre (commercial IF), almond shell, beet pulp, dry olive cake, and oat hull) and examined the effects of their soluble fractions on fimbriated (F4) and non-fimbriated (NF) E. coli. Intestinal porcine epithelial (IPEC-J2) cells were used as an in vitro model. Almond shell and commercial IF contained more insoluble and total dietary fibre (p < 0.001), while wheat bran contained the least total dietary fibre (p < 0.001). Beet pulp contained the most soluble fibre and had the highest water holding capacity (p < 0.001). Commercial IF had the highest swelling capacity (p < 0.001) and dry olive cake the highest cold solubility (p < 0.001). Based on adherence assays, wheat bran, oat hull, almond shell, and dry olive cake significantly prevented E. coli F4 from adhering to IPEC-J2 cells, whereas for NF E. coli, only dry olive cake reduced bacterial infection (p < 0.05). Dry olive cake was the most effective in reducing expression of proinflammatory cytokines, predominantly interleukin 8 (IL-8) and tumour necrosis factor-α (TNF-α) (p < 0.001). In conclusion, aqueous extracts of wheat bran, oat hull, almond shell, and dry olive cake contain compounds that interfere with E. coli F4 adherence to IPEC-J2 cells.
- PublicationRestrictedPlatelet glycoprotein Ibα supports experimental lung metastasis(National Academy of Sciences, 2007-05-22) Guerrero López, José Antonio; Jain, Shashank; Zuka, Masahiko; Liu, Jungling; Russell, Susan; Dent, Judith; Forsyth, Jane; Maruszak, Brigid; Gartner, T. Kent; Felding-Habermann, Brunhilde; Ware, Jerry; Medicina Interna; Facultad de MedicinaThe platelet paradigm in hemostasis and thrombosis involves an initiation step that depends on platelet membrane receptors binding to ligands on a damaged or inflamed vascular surface. Once bound to the surface, platelets provide a unique microenvironment supporting the accumulation of more platelets and the elaboration of a fibrin-rich network produced by coagulation factors. The platelet-specific receptor glycoprotein (GP) Ib-IX, is critical in this process and initiates the formation of a platelet-rich thrombus by tethering the platelet to a thrombogenic surface. A role for platelets beyond the hemostasis/thrombosis paradigm is emerging with significant platelet contributions in both tumorigenesis and inflammation. We have established congenic (N10) mouse colonies (C57BL/6J) with dysfunctional GP Ib-IX receptors in our laboratory that allow us an opportunity to examine the relevance of platelet GP Ib-IX in syngeneic mouse models of experimental metastasis. Our results demonstrate platelet GP Ib-IX contributes to experimental metastasis because a functional absence of GP Ib-IX correlates with a 15-fold reduction in the number of lung metastatic foci using B16F10.1 melanoma cells. The results demonstrate that the extracellular domain of the α-subunit of GP Ib is the structurally relevant component of the GP Ib-IX complex contributing to metastasis. Our results support the hypothesis that platelet GP Ib-IX functions that support normal hemostasis or pathologic thrombosis also contribute to tumor malignancy.
- PublicationOpen AccessValidez y confiabilidad de un instrumento evaluativo de adherencia en pacientes con falla cardiaca(Murcia : Servicio de Publicaciones de la Universidad de Murcia, 2012) Achury Saldaña, Diana; Sepúlveda Carrillo, GJ.; Rodríguez Colmenares, SM.; Giraldo, IC.Se describe la construcción y validación de un instrumento para evaluar la adherencia al tratamiento en pacientes con falla cardiaca, usando como referencia teórica los factores asociados con la adherencia y sus respectivos indicadores taxonomía NOC. Consta de 30 ítems agrupados en diez dimensiones. El Objetivo fue establecer los indicadores iniciales de fiabilidad y validez de constructo del instrumento de “evaluación de comportamientos de adherencia al tratamiento farmacológico y no farmacológico en pacientes con falla cardíaca”. El instrumento fue aplicado a 192 personas con diagnóstico de falla cardíaca y se estableció la validez de constructo mediante análisis factorial y la consistencia interna mediante la prueba estadística Alfa de Cronbach. El análisis psicométrico de este estudio mostró un índice de fiabilidad de 0,7213 lo que se considera adecuado, este instrumento se convierte en una herramienta de valoración de enfermería que puede ser útil para evaluar la adherencia al tratamiento en pacientes con falla cardíaca.