Browsing by Subject "Acute phase response"

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    Alterations in haemolymph proteome of Mytilus galloprovincialis mussel after an induced injury
    (Elsevier, 2018-01-31) Franco-Martínez, Lorena; Martínez-Subiela, Silvia; Escribano, Damián; Schlosser, Sarah; Nöbauer, Katharina; Razzazi-Fazeli, Ebrahím; Romero, Diego; Cerón, José Joaquín Cerón; Tvarijonaviciute, Asta; Ciencias Sociosanitarias
    A proteomic and biochemical approach was performed to assess the effects of an induced muscle injury on the haemolymph of bivalve molluscs. For this purpose, Mytilus galloprovincialis were exposed to puncture of adductor muscle for three consecutive days, and their haemolymph proteome was then compared to healthy animals using 2-dimensional electrophoresis (2-DE) to identify proteins that differed significantly in abundance. Those proteins were then subjected to tandem mass spectrometry and 6 proteins, namely myosin, tropomyosin, CuZn super-oxide dismutase (SOD), triosephosphate isomerase, EP protein and small heat shock protein were identified. SOD and tropomyosin changes were verified by spectrophotometric measurements and western blotting, respectively. As some of the proteins identified are related to muscular damage and oxidative stress, other biomarkers associated with these processes that can be evaluated by automatic biochemical assays were measured including troponin, creatine kinase (CK), and aspartate aminotransferase (AST) for muscle damage, and SOD, trolox equivalent antioxidant capacity (TEAC) and esterase activity (EA) for oxidative stress. Significantly higher concentrations of troponin, CK, AST, and TEAC were observed in mussels after puncture, being also possible biomarkers of non-specific induced damage.
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    Changes in serum biomarkers of inflammation in bovine besnoitiosis
    (2021-09-22) González Barrio, David; Huertas López, Ana; Diezma Díaz, Carlos; Ferre, Ignacio; Cerón Madrigal, José Joaquín; Ortega Mora, Luis Miguel; Álvarez García, Gema; Medicina y Cirugía Animal
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    Evaluation of C-reactive-like protein in Mytilus galloprovincialis
    (Elsevier, 2019-07-05) Franco-Martínez, Lorena; Tvarijonaviciute, Asta; Mateo, Sandra V.; Cerón, José J.; Romero, Diego; Oliveira, Miguel; Teles, Mariana; Martínez-Subiela, Silvia; Ciencias Sociosanitarias
    C-reactive protein (CRP) is an acute phase protein of the innate immune system with an important role as biomarker in many biological processes in mammals. However, there is a lack of information regarding CRP in molluscs and there is no data about the presence of CRP-like protein in mussels. In the present work, CPR-like protein was identified and purified from the digestive gland of the Mediterranean mussel Mytilus galloprovincialis In addition, it was demonstrated that CRP-like protein can be measured accurately and precisely by a high- throughput automated method. Finally, higher levels of CRP-like protein in mussels exposed to hypoxia and nanoplastics were detected when compared to controls. The results obtained indicate that CRP-like protein could be a suitable novel biomarker of inflammatory response in mussels for biomonitoring programs being economic, easy and rapid to determine.