Browsing by Subject "Acute phase protein"

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    Acute phase protein concentrations in retired racing Greyhounds
    (Wiley, 2009-06) Guillermo Couto, C.; Cerón Madrigal, José Joaquín; Parra Muñoz, María Dolores; Martínez-Subiela, Silvi; Lazbik, M. Cristina; Ciencias Sociosanitarias
    Background: Retired racing Greyhounds are popular as pets. Greyhounds have several differences in physiological values compared with other breeds, including lower serum a- and b-globulin concentrations. We hypothesized that lower acute phase protein (APP) concentrations could contribute to lower a- and b-globulin concentrations in this breed. Objectives: The purpose of this study was to compare serum concentrations of several APPs in Greyhounds with those of other dog breeds. Methods: We measured the serum concentrations of C-reactive protein (CRP), haptoglobin (Hp), acid-soluble glycoprotein (ASG), ceruloplasmin (CP), and serum amyloid A (SAA) in 15 clinically healthy retired racing Greyhounds and 11 age- and gender-matched healthy nonGreyhound controls using previously validated methods. Results were compared by Student’s t-tests. Results: The concentration of Hp by both colorimetric and immunoturbidimetric methods was significantly lower in Greyhounds than in nonGreyhound dogs (P =.0009 and .019, respectively). The concentration of ASG was also significantly (P =.007) lower in Greyhounds, but CRP and CP concentrations were not significantly different between groups. SAA concentration was below the detection limit of the method in all dogs. Conclusions: The low serum concentrations of Hp and ASG should be taken into consideration when interpreting APP results in Greyhounds. Because both Hp and some ASG migrate in the a-globulin fraction, these results may explain the low a-globulin concentrations in Greyhounds.
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    Serum paraoxonase type-1 activity in pigs: assay validation and evolution after an induced experimental inflammation
    (Elsevier, 2015-02-15) Escribano Tortosa, Damián; Tvarijonaviciute, Asta; Tecles Vicente, Fernando; Cerón Madrigal, José J.; Producción Animal
    Paraoxonase 1 (PON1) is a serum enzyme synthesised and secreted primarily by the liver. It possesses anti-inflammatory properties limiting the production of pro-inflammatory mediators. The objectives of this study were to validate three spectrophotometric assays for the quantification of PON1 activity in pig serum, and to determine if PON1 activity in porcine behaves as a negative acute phase protein (APP), decreasing in inflammatory conditions. An analytical validation using three different substrates – 5-thiobutil butyrolactone (TBBL), phenylacetate (PA) and 4-(p)-nitrophenyl acetate (pNA) – was performed. In addition, inflammation was experimentally induced in five pigs by subcutaneous injection of turpentine oil, while five control pigs were left untreated. The treated pigs showed significant increases in CRP and decreases in albumin, indicating an inflammatory condition. The three substrates used would be suitable for PON1 activity measurements in serum samples, since they offer adequate precision (coefficients of variation < 10%), sensitivity (0.01, 0.15, 0.02 U/mL for TBBL, pNA and PA respectively) and accuracy (r = 0.99). In addition, PON1 behaves as a negative APP in pigs since a significant decrease (P < 0.05) in its activity after 72 h of the induction of the inflammation was observed with all substrates.