Browsing by Subject "ARPE-19 cells"
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- PublicationRestrictedPigment epithelium-derived factor protects retinal pigment epithelial cells against cytotoxicity “in vitro”(Springer Nature., 2018-05-03) Nadal-Nicolás, Francisco Manuel; Becerra, S. Patricia; Oftalmología, Optometría, Otorrinolaringología y Anatomía Patológica; Facultad de MedicinaOxidative stress has been implicated in neurodegenerative diseases, such as age-related macular degeneration. Hydrogen peroxide and sodium iodate can mediate oxidative injury. Sodium iodate induces a selective retinal degeneration targeting the RPE. We describe a method of chronic sodium iodate-mediated injury on RPE cells that may serve to evaluate protective factors against oxidative stress. Cytotoxicity and cell viability curves of ARPE-19 cells with sodium iodate were generated. The antioxidant pigment epithelium-derived factor decreased sodium iodate-mediated cytotoxicity without affecting ARPE-19 cell viability. A cell culture system to evaluate protection against oxidative stress injury with PEDF is discussed.