Datos de Investigación

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https://hdl.handle.net/10201/37686
El objetivo de esta comunidad es poner a disposición de los usuarios aquellos materiales generados o recolectados durante el transcurso de una investigación.

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Browsing Datos de Investigación by Subject "Anthropogenic stress"

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    Cellular responses to electromagnetic fields and ultraviolet radiation: an in vitro study on microalgae and fish cell lines
    (Elsevier, 2026) Ortiz García, María; Terrer Marcilla de Teruel Moctezuma, José María; García Gamuz, José Antonio; Esteban Abad, María de los Ángeles; Aboal Sanjurjo, Marina; Biología Celular e Histología; María Ángeles Esteban Abad; Facultades de la UMU::Facultad de Biología
    Background The molecular effects involved in the cellular response to non-ioninzing electromagnetic radiation on marine and freshwater microalgae and fish cell lines are incompletely understood. Objectives The direct impact of non-ionizing electromagnetic radiation on marine and freshwater microalgae and fish cell lines was investigated. Methods Six microalgae species from different taxonomic groups were exposed to extremely low-frequency electromagnetic fields (ELF-EMF) for five days. Four fish cell lines (SAF-1, DLB-1, DLEC, and Fugu eye) were exposed to ultraviolet (UV) radiation, with SAF-1 demonstrating the highest sensitivity. In addition, three commercial antioxidants (2101, 3600, and 4004) were tested for their possible radioprotective effects. Results Although no statistically significant differences in cell density were observed, certain taxa (Chlorotylium sp., Mayamea sp., and Scenedesmus sp.) exhibited a declining trend compared to the controls, suggesting species-specific sensitivity to ELF-EMF. Principal component analysis revealed significant differences in physiological responses, particularly in photosynthetic variables such as electron transport rate and dissolved carbon. The total antioxidant capacity did not differ significantly between the control and exposed microalgae, and Chlorotylium sp. showed the highest basal levels. The photosystem II productivity index remained largely unaffected by ELF-EMF exposure, except for an increase in irradiated Scenedesmus sp. Low antioxidant concentrations (≤125 µg/ml) and short incubation times (6 h) of 2101 and 3600 induced proliferation or increased cell viability, particularly under irradiation. However, prolonged incubation time (24 h) or high antioxidant concentrations (≥250 µg/ml) resulted in significant cytotoxicity in all cell lines.