Informes o documentos de trabajo

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Open Access
Nuestros barrios. Revista de los jóvenes de Murcia.
(Coordinadora de Vocalías y Clubs Juveniles, 1978) Reverte Martínez, Francisco Manuel; Sanchez, Francisco Luis; Ripoll Spiteri, Antonio; Derecho Civil
Revista de los jóvenes de la ciudad de Murcia desde la realidad de sus barrios y en reivindicacion de los derechos juveniles en la transición democrática española
Open Access
"A toda marcha". Periódico Juvenil de Vistabella
(Vocalía Juventud. Asociacion de Vecinos de Vistabella., 1980-05) Balibrea Sánchez, Javier; Reverte Martínez, Francisco Manuel; Lidón Cerezuela, María Beatriz; Derecho Civil
Revista juvenil del barrio de Vistabella, Murcia, con artículos sobre musica, cultura, mujer, deporte, y reivindicaciones juveniles de la época, 1980.
Open Access
La plaza de los patos. Revista del barrio de Vistabella. Murcia.
(Jóvenes de Vistabella, 1983-04) Olcina Bo, Francisco; Reverte Martínez, Francisco Manuel; Gil de Pareja Perona, Narciso; Derecho Civil
Revista de los jóvenes del barrio de Vistabella, Murcia, con cuestiones culturales, sociales, y recreativas, reflejando las circunstancias y problemáticas de los jóvenes en los primeros años de la década de los 80.
Open Access
Casas de Juventud de Murcia
(Ayuntamiento de Murcia. Concejalía de Juventud., 1985) Reverte Martínez, Francisco Manuel; Martínez Gómez, Rosa María; Cases García, Ricardo; Derecho Civil
Publicación que presenta el proyecto de Casas de Juventud de Murcia, sus fines y objetivos, bases jurídico - administrativas y fórmulas de gestión conjunta entre asociaciones y grupos de jóvenes y la Concejalía de Juventud del Ayuntamiento de Murcia.
Open Access
1987.Memoria de los espacios jóvenes Yesqueros y La Nave
(Ayuntamiento de Murcia., 1987) Reverte Martínez, Francisco Manuel; Jular Pérez-Alfaro, Teresa; Poveda Sainz, Emilio; Derecho Civil
Memoria evaluación del primer año de funcionamiento de los Espacios Jóvenes Municipales de Murcia, YESQUEROS Y LA NAVE, actividades, recursos y diseños gráficos.
Open Access
Recombinant Vaccine for Canine Parvovirus in Dogs
(American Society for Microbiology, 1992-02-07) López de Turiso, José Ángel; Cortés, Elena; Martínez, Concepción; Ruiz de Ybáñez Carnero, María del Rocío; Simarro, Isabel; Vela, Carmen; Casal, Ignacio; Sanidad Animal
VP2 is the major component of canine parvovirus (CPV) capsids. The VP2-coding gene was engineered to be expressed by a recombinant baculovirus under the control of the polyhedrin promoter. A transfer vector that contains the lacZ gene under the control of the plO promoter was used in order to facilitate the selection of recombinants. The expressed VP2 was found to be structurally and immunologically indistinguishable from authentic VP2. The recombinant VP2 shows also the capability to self-assemble, forming viruslike particles similar in size and appearance to CPV virions. These viruslike particles have been used to immunize dogs in different doses and combinations of adjuvants, and the anti-CPV responses have been measured by enzyme-linked immunosorbent assay, monolayer protection assays, and an assay for the inhibition of hemagglutination. A dose of ca. 10 ,ug of VP2 was able to elicit a good protective response, higher than that obtained with a commercially available, inactivated vaccine. The results indicate that these viruslike particles can be used to protect dogs from CPV infection.
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Identification of types of canine parvovirus circulating in Spain
(Wiley, 1995-02-18) Ruiz de Ybáñez Carnero, María del Rocío; Vela, Carmen; Cortés, Elena; Simarro, Isabel; Casal, J. Ignacio; Sanidad Animal
From 1990 to 1993, the presence of canine parvovirus (CPV) was confirmed in 13 of 141 samples of faeces or intestinal contents from dogs in Spain with clinical signs of CPV infection. Typing of the samples using an indirect double antibody sandwich ELISA (ELISA-IDAS) showed that 8 samples, including 3 obtained in 1993, were classified as CPV-2a and 5 as CPV-2b. An in vitro neutralization assay showed that 4 of a panel of 6 neutralizing monoclonal antibodies (mAbs) neutralized the original CPV-2 type, CPV-2a and CPV-2b completely while another produced the same level of partial neutralization of all isolates. One mAb produced complete neutralization of CPV-2 but only partial neutralization of CPV-2a and CPV-2b. This is the first report of the isolation of different CPV antigenic types in Spain and outside the USA. The results indicate clearly that these mutations will affect neither control of the disease by vaccination (since the critical neutralization epitopes are not affected) nor the diagnosis of the disease by current serological methods.
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Evaluation of the prevalence of sarcoptic mange in slaughtered fattening pigs in southeastern Spain
(Elsevier, 1998-04-15) Alonso de Vega, Francisco; Mendez de Vigo, J.; Ortiz Sánchez, Juana; Martínez-Carrasco Pleite, Carlos; Albaladejo Serrano, Antonio; Ruiz de Ybáñez Carnero, María del Rocío; Sanidad Animal
In this study the prevalence of sarcoptic mange in fattening pigs in Murcia, southeastern Spain was investigated. Results showed that 37% of the 1318 slaughtered pigs examined were positive for Sarcoptes scabiei var. suis. Skin lesions potentially attributable to this mite were present in 92.80% of animals, but the parasite could be detected in only 38.60% of them. The condition of the ear cleanrdirty. was not a defining characteristic for the diagnosis of this swine disease. A sucrose flotation–concentration technique was more effective than direct microscopy in finding the mite in ear scrapings
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Intestinal myiasis in a dog
(Wiley, 1998-10-24) Manchón, Manuel; Ruiz de Ybáñez Carnero, María del Rocío; Alonso de Vega, Francisco; Sanidad Animal
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Sarcoptic mange in Spanish ibex from Spain
(Wildlife Disease Association, 1999) León-Vizcaíno, Luis; Ruiz de Ybáñez Carnero, María del Rocío; Cubero, Maria J.; Ortiz, Juana M.; Espinosa, Javier; Pérez, Linarejos; Simón, Miguel A.; Alonso, Francisco; Sanidad Animal
The Spanish ibex (Capra pyrenaica hispanica) population of the ‘‘Sierras de Cazorla, Segura y Las Villas’’ Nature Park (Spain) was isolated as the result of a severe epidemic of sarcoptic mange. In this context, the dynamic haracteristics of the disease were analyzed in a wild group consisting of 35 animals from the beginning of the epizootic (when the mating period started) to the extinction of the population due to mange. Monthly tracking permitted the sequential characterization of the pathology in each animal. The duration of the disease was 2 to mo, evolving to severe disease and terminating in death. Incidence and prevalence rates in terms of morbidity and severity, and mortality and lethality were calculated. At the end of the mating season, 81% of the population were affected. There were no statistically significant dif ferences in severity of the disease across sex or age categories of the animals. Most of the carcasses were found in caves used as refuge and/or near rivers or streams. Additionally, 46 of the 63 (73%) ibex captured in different areas of the nature park were naturally infected with the Sarcoptes scabiei. Infected ibex were examined for number of mites during the initial stage of the disease (n 5 3), in the development stage (n 5 12), in the consolidation stage (n 5 17), and in the chronic stage (n 5 14). The prevalence of mites in different anatomical regions was determined in each of these phases of the infection. A histological study of the skin lesions was conducted in 22 animals. Both the clinical and the pathological (macroscopic and icroscopic) aspects of the sarcoptic mange in Spanish ibex corresponded to the classic description of sarcoptic mange in other wild and domestic small ruminants.
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Improved methods for recovering eggs of Toxocara canis from soil
(Cambridge University Press, 2000-04-13) Ruiz de Ybáñez Carnero, María del Rocío; Garijo Toledo, María Magdalena; Goyena Salgado, Marina; Alonso de Vega, Francisco Domingo; Sanidad Animal
The ingestion of soil in parks and public places containing eggs of Toxocara may constitute a significant health risk, particularly to children. To determine the most efficient method for extracting eggs from experimentally contaminated soil, two consecutive studies were undertaken. Four techniques, including washing, sieving, vacuum, and the one recommended by the World Health Organization, were evaluated. Recovery rates of over 85% were recorded with both washing and sieving methods. Using the washing technique, all combinations of the four pre-treatment solutions, distilled water, acetoacetic solution pH 5, 0.1 N sodium hydroxide and 1% Tween 20, and seven flotation fluids with different speci®c gravities (S.G.) ranging from 1.20 to 1.35 were assayed. The association of distilled water and saccharose solution with an S.G. of 1.27 showed the best results, with a recovery rate of 99.91%.
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Use of pooled faecal samples in assessing nematode egg shedding in captive gazelles (Gazella species)
(Wiley, 2000-08-12) Ruiz de Ybáñez Carnero, María del Rocío; Ortiz, Juana; Abaigar, Teresa; Espeso, Gerardo; Cano, Mar; Sanidad Animal
Open Access
Oral administration of mebendazole failed to reduce nematode egg shedding in captive African gazelles
(AOSIS Publishing, 2000-11-24) Ortiz Sánchez, Juana; Ruiz de Ybáñez Carnero, María del Rocío; Abaigar, Teresa; Garijo, M. Magdalena; Espeso, Gerardo; Cano, Mar; Sanidad Animal
Idiosyncracies are observed in captive wild animals as regards the pharmacokinetics and efficacy of anthelmintics. This could be attributed to such factors as differences in host's metabolism, irregular distribution of anthelmintics due to the way they are administered and worm resistance to anthelmintics. Previously mebendazole was found to be poorly effective when administered in feed. An experiment was conducted to evaluate the efficacy of mebendazole when administered at the dosage rate of 15-20 mg/kg body weight to gastrointestinal nematodes in captive gazelles. Fifty-eight adult gazelles (Gazella cuvien) were divided into four groups: T1 (animals dosed orally, directly into the mouth), T2 (treated orally, mixed in the water of a herd), T3 (treated orally, mixed in the water of one animal), and T4 (not treated). Individual faecal samples were taken before treatment, and 15 days thereafter. Mean percentages of reduction of egg shedding were calculated for Nematadirus spp., other trichostrongyles, total trichostrongylids, Trichuris spp. and total nematodes. No statistically significant differences were detected between the treatment groups and the control group or among the animals in the three treatment groups
Open Access
First report of Trichostrongylus probolurus (Railliet, 1896) Loos, 1905 (Nematoda:Trychostrongyloidea) in Spain
(Asociación de Parasitólogos Españoles, 2000-12-01) Ortiz Sánchez, Juana; Ruiz de Ybáñez Carnero, María del Rocío; Goyena Salgado, Marina; Sanidad Animal
The gastrointestinalnematode Trichostrongylus probolurus has been identifiedin gazelles(Gazella dorcas and G. dama mhorr) in the Estaci6n Experimental de Zonas Aridas (e.S.I.e., Almerfa).This is the first report of the presence of the parasite both in G. dama mhorr and in Spain.
Embargo
Records of Eimeria spp. and their patterns of excretion in captive North African Gazelles
(Helminthological Society of Washington, 2001) Ruiz de Ybáñez Carnero, María del Rocío; Ortiz, Juana; Garijo Toledo, María Magdalena; Espeso, Gerardo; Abaigar, Teresa; Cano, Mar; Sanidad Animal
The species of Eimeria occurring in 3 species of captive gazelles (Gazella dama mhorr, Gazella cuvieri, and Gazella dorcas neglecta) were identified. This is the first report of Eimeria pallida, Eimeria elegans, and Eimeria gazella in these hosts and also the first report of E. elegans and E. gazella in Spain. Feces were collected from each of 9 young gazelles for periods of 3-7 mo to determine their oocyst shedding profile. Most oocysts appeared at 20-25 d, peaked, and decreased to undetectable levels between 40 and 115 d. Eimeria gazella delayed its excretion, which continued at low levels throughout the observation stage. No clinical signs of infection were observed in the gazelles during the study.
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Prevalence and viability of eggs of Toxocara spp. and Toxascaris leonina in public parks in eastern Spain
(Cambridge University Press, 2001-01-29) Ruiz de Ybáñez Carnero, María del Rocío; Garijo Toledo, María Magdalena; Alonso de Vega, Francisco; Sanidad Animal
To demonstrate the prevalence of Toxocara spp. and Toxascaris leonina eggs in parks in Murcia city, eastern Spain, a total 644 soil samples were examined from nine parks. More than 67% of parks and 1.24% of soil samples were contamined and the mean egg density per sample was 16:7 ^ 20 eggs per 100 g of soil. Over 97% of eggs identified were viable. Only one sample was positive for Toxascaris leonina. The present findings suggest that shady conditions are important for the occurrence and viability of Toxocara spp. and Toxascaris leonina eggs in soil as there were significantly more positive samples in shaded and moist areas compared with open and dry habitats.
Open Access
Voga & Care Manual
(2001-06-01) Angosto Sánchez, Salvador; Borrego Balsalobre, Francisco José; Actividad Física y Deporte; Facultades de la UMU::Facultad de Ciencias del Deporte
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Effect of different methods of administration of ivermectin on its efficacy against the shedding of gastrointestinal nematode eggs by gazelles
(Wiley, 2001-07-07) Ortiz Sánchez, Juana; Ruiz de Ybáñez Carnero, María del Rocío; Abaigar, Teresa; Goyena, Marina; Espeso, Gerardo; Cano, Mar; Alonso de Vega, Francisco; Sanidad Animal
Seven different methods of administering ivermectin to gazelles were compared: subcutaneous injection, direct oral administration, administration in individual feeds, administration in a herd feed, direct oral administration of a second ivermectin formulation, administration in individual water supplies, and administration in the herd's water supply. The first five treatments were effective, as monitored by faecal egg count reduction tests, and administration in individual feeds or in a herd feed avoided the need to capture the animals, with the attendant risk of mortality. Of the factors associated with the recipients (species, sex, age and inbreeding coefficient) age was the only significant factor for the efficacy of the treatment. Oral or subcutaneous, individual or collective, and direct or indirect administrations were equally satisfactory for the treatment of all the parasite groups studied. Only when parasitic problems were due to Nematodirus species did direct administration to individual animals appear to be preferable.
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Experimental ivermectin treatment of sarcoptic mange and establishment of a mange-free population of spanish ibex
(Wildlife Disease Association, 2001-10) León Vizcaíno, Luis; Cubero, M. José; González Capitel, Emilio; Simón, Miguel Ángel; Pérez, Linarejos; Ruiz de Ybáñez Carnero, María del Rocío; Ortiz Sánchez, Juana; González Candela, Mónica; Alonso de Vega, Francisco; Sanidad Animal
Ivermectin was used to treat sarcoptic mange in Spanish ibex (Capra pyrenaica hispanica). Its therapeutic effectiveness was analyzed when it was administered through subcutaneous injection, to sick animals in the consolidation stage of mange (third phase) and, with double injections to chronically affected animals (fourth phase) at a dosage of 0.2 or 0.4 mg/kg body weight (bw). Three wk after treatment, the animals in the third phase of mange treated with a high dose (0.4 mg/kg bw) of ivermectin were completely cured. The same result was achieved after 4 wk of treatment in those animals in phase 3 of mange when 0.2 mg/kg body weight was used. Double injection with ivermectin, even at high doses, did not guarantee the complete cure of all cases of sarcoptic mange in the chronic stage (phase 4); only three of six animals were free of Sarcoptes scabiei. The second experiment consisted on the application of a sanitation program in order to obtain a population of Spanish ibex free from S. scabiei, starting with free-ranging animals, some of them healthy and others sick. After capture the animals were classified as chronically ill, in which case they were excluded from the program, mite carriers and healthy specimens. All the animals were treated first topically with foxim (500 mg/l) and subcutaneously with ivermectin (0.4 mg/kg bw). The infected animals were housed in the treatment pen, and received two doses of ivermectin (0.2 mg/kg bw) at an interval of 15 days, then spent 15 days in the quarantine pen, where they received a further dose before they were included in the pool of healthy animals, and immediately were placed in the quarantine phase. The sanitation we implemented was fully effective in curing the affliction of Spanish ibex affected by S. scabiei
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Abomasal and small intestinal nematodes from captive gazelles in Spain
(Cambridge University Press, 2001-12) Ortiz Sánchez, Juana; Ruiz de Ybáñez Carnero, María del Rocío; Garijo Toledo, María Magdalena; Goyena, Marina; Espeso, Gerardo; Abáigar, Teresa; Cano, Mar; Sanidad Animal
The abomasal and small intestinal helminth fauna of three species of captive gazelles (Gazella dama mhorr, G. cuvieri and G. dorcas neglecta) kept in captivity in AlmerõÂa (southeast Spain) have been studied, and the following species were identified: Nematodirus spathiger, N. filicollis, N. helvetianus, Camelostrongylus mentulatus, Trichostrongylus vitrinus, T. probolurus, T. colubriformis, Ostertagia ostertagi, O. harrisi, Teladorsagia (Ostertagia) circumcincta, and T. (Ostertagia) davtiani. Camelostrongylus mentulatus and N. spathiger were the most prevalent and abundant parasites. Ostertagia ostertagi, O. harrisi, N. helvetianus, and T. (Ostertagia) davtiani were identified for the first time in the genus Gazella. In addition, O. harrisi and Trichostrongylus probolurus are new records for Spain