Pachymic acid ameliorates polycystic ovary syndrome via inactivating Akt/ERK signaling

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dc.contributor.authorChunhua Zhang
dc.contributor.authorFang Fang
dc.contributor.authorYuanyuan Yi
dc.contributor.authorDongmei Ji
dc.contributor.departmentBiologĂ­a Celular e HistologĂ­a
dc.date.accessioned2025-12-19T12:00:09Z
dc.date.available2025-12-19T12:00:09Z
dc.date.issued2026
dc.description.abstractObjective. Polycystic ovary syndrome (PCOS) is a common reproductive endocrine disorder that adversely affects women’s health and quality of life. Pachymic acid (PA), a bioactive ingredient from Poria cocos (Schw.) Wolf, has demonstrated protective effects against PCOS in a murine model. This study aims to investigate the underlying mechanism by which PA exerts protective effects against PCOS. Methods. Female Sprague-Dawley rats were treated with letrozole to induce PCOS. The ovarian granulosa cell line (KGN) was exposed to lipopolysaccharide (LPS) to mimic PCOS in vitro. Hematoxylin-eosin staining and TUNEL assay were used for ovarian histological analysis. The cell counting kit-8 assay was used to assess the viability of KGN cells. Flow cytometry was used for in vitro cell apoptosis analysis. Western blotting revealed molecular protein expression levels in rat ovaries and KGN cells. Results. PA attenuated LPS-induced lactate dehydrogenase release (p<0.01), reduced the cell apoptosis rate (p<0.001), Bax, and cleaved-caspase3 protein expression (p<0.001), and increased Bcl-2 protein expression (p<0.01) in KGN cells. PA attenuated letrozole-induced increases in testosterone (p<0.01), luteinizing hormone (p<0.01), and estradiol levels (p<0.05) and decreases in progesterone levels (p<0.05) in PCOS rats. PA promoted corpus luteum formation (p<0.001) and reduced the number of cystic follicles and cell apoptosis (p<0.001) in PCOS rats. PA blocked Akt and ERK signaling transduction in PCOS rats and KGN cells (p<0.001). Conclusion. PA protects against PCOS and attenuates cell apoptosis by inactivating Akt and ERK signaling.
dc.formatapplication/pdf
dc.format.extent9
dc.identifier.doihttps://doi.org/10.14670/HH-18-931
dc.identifier.eissn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/181689
dc.languageeng
dc.publisherUniversidad de Murcia, Departamento de Biologia Celular e Histiologia
dc.relationSin financiacion externa a la Universidad
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectPachymic acid
dc.subjectAkt/ERK signaling
dc.subjectPolycystic ovary syndrome
dc.subject.odsNo relacionado con ningĂşn objetivo de desarrollo sostenible
dc.titlePachymic acid ameliorates polycystic ovary syndrome via inactivating Akt/ERK signaling
dc.typeinfo:eu-repo/semantics/article
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Histology and histopathology, Vol.41, NÂş1, (2026)