Publication: Transposon-based reprogramming to induced pluripotency
Authors
Kumar, Dharmendra ; Talluri, Thirumala R. ; Anand, Taruna ; Kues, Wilfried A.
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Publisher
Universidad de Murcia. Departamento de BiologĂa Celular e HistologĂa
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DOI
10.14670/HH-11-656
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info:eu-repo/semantics/article
Description
Abstract
Induced pluripotent stem (iPS) cells
represent a recent innovation in the field of stem cells.
Commonly, iPS cells are generated by viral transduction
of core reprogramming genes, such as Oct4, Sox2, Klf4,
c-Myc, Nanog and Lin28. However, integrating viruses,
like retro- and lentiviral vectors, may cause insertional
mutagenesis and may increase the risk of tumor
formation. Therefore, alternative methods which avoid
these safety concerns are intensively investigated. Here,
we review the current status of transposon-based
methods to induce pluripotency. DNA transposons are
non-viral elements, which can be effectively integrated
into a genome by their corresponding transposase
enzyme. The advantages of transposon-based gene
transfer are their increased safety, their large cargo
capacity, their relatively simple design, and the
availability of hyper-active and mutated transposase
enzymes. For example, integration-deficient, excisioncompetent transposase variants allow the complete
removal of the reprogramming transposon after
successful reprogramming to obtain transposon-free
reprogrammed cells. Transposon-based reprogramming
broaden the toolbox for iPS cell production and will
advance the establishment of safe, non-viral methods.
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Citation
Histology and Histopathology, vol.30, nÂş 12
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