Publication: Morphometric analysis of bromodeoxyuridine
distribution and cell density in the rat Dunning
prostate tumor R3327-AT1 following treatment
with radiation andtor hyperthermia
Loading...
Date
1999
Authors
Peschke, P. ; Klein, V. ; Wolber, G. ; Friedrich, E. ; Hahn, E.W.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
To monitor cellular response to single doses
of radiation (RT) andlor local tumor hyperthermia (LTH)
proliferation kinetics were determined in the anaplastic
prostate adenocarcinoma R3327-AT1 grown in
Copenhagen rats. Tumor-bearing animals were injected
i.v. with a bolus of bromodeoxyuridine (BrdUrd), and at
defined times after treatment the tumors were surgically
removed, fixed and embedded in paraffin. BrdUrd
incorporated into the DNA of S-Phase nuclei was
detected on 4-6 pm-thick tissue sections using a monoclonal
anti-BrdUrd antibody followed by streptavidinbiotin
and alkaline phosphatase as a reporter system.
Cell nuclei were stained with the fluorescence dye DAPI
(Diaminophenylindole). Morphometric analysis was
performed using a computer-assisted Leitz-TASIplus
system. Depending on tumor size, up to 18,000
nuclei were routinely analyzed. Untreated tumors of
standardized size (8-10 mm) exhibited a BrdUrdlabeling
index (LI) of (6.921.6)%. In general, the L1 was
higher in the periphery than in the center, being more
pronounced in larger tumors. After 6 Gy y-rays, the
mean L1 decreased to 1.8% (24 h) and rose afterwards to
5.4% by 168 h. Following LTH (43.5 "C, 35 min water
bath), the mean L1 rapidly decreased to 2% (8 h), rose to
9.8% (48 h), and plateaued at 6% after 168 h. A
combined treatment consisting of irradiation (6 Gy)
followed by LTH yielded smallest L1 (2.4+0.18%) and
lowest cell density (111+0.6 nuclei per field) by 168 h.
The morphometric procedure was reliable and reproducible
and can be used to characterize and compare the
effects of different therapies on cell kinetics. Of
particular value is that these analyses are done on an
Offprint requests to: Dr. Peter Peschke. Deutsches Krebsforschungszentrum,
Forschungsschwerpunkt Radiologie. Im Neuenheimer Feld
280, 69120 Heidelberg, Germany. Fax:+ 6221-422572. e mail:
P.Peschke@DKFZ-HeideIberg.de
intact tissue architecture and hence enable a better
interpretation of flow cytometric results of treatmentinduced
alterations within different topohistological
regions in solid tumors. Moreover, the technique
provides the basis for 3D reconstruction of the cellular
activity and heterogeneity of experimental neoplasms.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.