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Development of an industrial sustainable process for wax esters production: enzyme immobilization, process optimization, and plant simulation

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dc.contributor.authorMontiel, M.C.
dc.contributor.authorSerrano-Arnaldos, M.
dc.contributor.authorYagüe, C.
dc.contributor.authorOrtega Requena, Salvadora
dc.contributor.authorMáximo, F.
dc.contributor.authorBastida, J.
dc.contributor.departmentIngeniería Química
dc.date.accessioned2024-02-01T15:18:00Z
dc.date.available2024-02-01T15:18:00Z
dc.date.issued2023-06-10
dc.description.abstractBACKGROUND: As a result of the ban on whaling, there has been a shortage of spermaceti, a natural product with applications in cosmetics and pharmaceuticals. A green and sustainable process for the biocatalytic synthesis of a wax ester mixture analogue to natural spermaceti is presented in this paper. RESULTS: Immobilization of Candida antarctica lipase B by covalent binding to Purolite® Lifetech™ ECR8285 allowed for an immobilized derivative with 124.5 mg protein/g support (69.3% protein immobilization yield) that maintains 100% of its enzymatic activity after 12 months of cold storage and presents negligible loses of activity after 9 consecutive reaction cycles. The optimization of the synthesis process in a batch reactor resulted in conditions that, at 70 °C, 350 rpm, and 1.25% w/w of biocatalyst, achieved a conversion of 97% after 1 h of reaction. The simulation of a spermaceti production plant was carried out using the process simulation software aspenONE suite v10. The plant was designed for a continuous operation during 9 h per day, with a reactor of 20.25 L of working volume and a residence time of 1 h. The production of this plant would be 173.25 kg spermaceti/day, with a product purity of 99.55%. CONCLUSION: The main novelty of this work is the design of a spermaceti production plant, using the most sustainable methodologies and resulting in a product with exceptional characteristics and minimal waste generation. Moreover, a new lipase immobilized derivative is also described. The good values of sustainability indicators point to the viability of its industrial implementation.es
dc.formatapplication/pdfes
dc.format.extent10es
dc.identifier.citationJournal of Chemical Technology and Biotechnology
dc.identifier.doihttps://doi.org/10.1002/jctb.7462
dc.identifier.issn0268-2575
dc.identifier.issn1097-4660
dc.identifier.urihttp://hdl.handle.net/10201/138450
dc.languageenges
dc.relationOrganismo Financiador: Ministerio de Ciencia, Innovación y Universidades. Convocatoria: PROYECTOS DE I+D+i «RETOS INVESTIGACIÓN» 2018, Proyecto: PROCESOS SOSTENIBLES PARA LA SINTESIS BIOCATALITICA DE ESTERES RAMIFICADOS. Código: RTI2018-094908-B-I00es
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/epdf/10.1002/jctb.7462es
dc.rightsAttribution-NonCommercial-NoDerivates 4.0 International
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCalB lipasees
dc.subjectEnzyme immobilizationes
dc.subjectGreen metricses
dc.subjectPlant simulationes
dc.subjectProcess optimizationes
dc.subjectWax esterses
dc.titleDevelopment of an industrial sustainable process for wax esters production: enzyme immobilization, process optimization, and plant simulationes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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