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Automated quantification and topographical distribution of the whole population of S- and L-cones in adult albino and pigmented rats

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dc.contributor.authorOrtín Martínez, Arturo
dc.contributor.authorJiménez López, Manuel
dc.contributor.authorSauvé, Yves
dc.contributor.authorVillegas Pérez, María Paz
dc.contributor.authorVidal Sanz, Manuel
dc.contributor.authorAgudo Barriuso, Marta
dc.contributor.authorSalinas Navarro, Manuel Ángel
dc.contributor.authorNadal-Nicolás, Francisco Manuel
dc.contributor.authorAlarcón Martínez, Luis
dc.contributor.departmentAnatomía Humana y Psicobiología
dc.date.accessioned2025-01-20T12:12:02Z
dc.date.available2025-01-20T12:12:02Z
dc.date.issued2010-06
dc.description© 2010 The Association for Research in Vision and Ophthalmology, Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Published Manuscript version of a Published Work that appeared in final form in Investigative Ophthalmology & Visual Science. To access the final edited and published work see
dc.description.abstractPurpose.: To quantify the whole population of S- and L-cones in the albino (Sprague-Dawley, SD) and pigmented (Piebald Virol Glaxo, PVG) rats and to study their topographical distribution within the retina. Methods.: Retinal radial sections and whole-mounted retinas were double immunodetected with antibodies against UV-sensitive and L-opsins to detect the S- and L-cones, respectively. Two automated routines were developed to quantify the whole population of S- and L-cones. Detailed isodensity maps of each cone type were generated. In both strains, the presence of dual cones was detected, these were semiautomatically quantified and their distribution determined. The matching distribution of retinal ganglion cells (RGCs) and L-cones was attained by double immunodetection of Brn3a and L-opsin, respectively Results.: The mean number ± SEM of L- or S-cones in SD and PVG retinas was 231,736 ± 14,517 and 239,939 ± 6,494 or 41,028 ± 5,074, and 27,316 ± 2,235, respectively. There was an increasing gradient of S-cone density along the inferonasal quadrant, although the highest densities were found in the retinal rims. The distribution of L-cones seemed to be complementary to the S-cones. The highest densities were observed in the superior nasotemporal axis, paralleling the distribution of Brn3a-positive RGCs. Conclusions.: These data establish, for the first time, the total number and the topographical distribution of S- and L-cones in two rat strains and demonstrate the correlation of L-cones and RGC spatial distribution.es
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dc.identifier.citationInvestigative Ophthalmology & Visual Science, 2010, Vol. 51, Issue 6, pp. 3171-3183.
dc.identifier.doihttps://doi.org/10.1167/iovs.09-4861
dc.identifier.issnPrint: 0146-0404 1552-5783
dc.identifier.issnElectronic: 1552-5783
dc.identifier.urihttp://hdl.handle.net/10201/148825
dc.languageenges
dc.publisherAssociation for Research in Vision and Ophthalmology
dc.relationSupported by Research Grants 05703/PI/07 and 04446/GERM/07from Fundación Séneca; FFIS-09/SMS01/BECA-08 Fundación para la Formación e Investigación Sanitarias de la Región de Murcia; SAF-2005-04812 Spanish Ministry of Education and Science; Spanish Ministry of Health and ISCIII-FEDER; PIO06/0780 and RD07/0062/0001, Red Temática de Investigación Cooperativa en Oftalmología.es
dc.relation.publisherversionhttps://iovs.arvojournals.org/article.aspx?articleid=2186407
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleAutomated quantification and topographical distribution of the whole population of S- and L-cones in adult albino and pigmented ratses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
relation.isAuthorOfPublication81da925b-fa46-4a1a-96fd-ce35568fa423
relation.isAuthorOfPublicationba9bf053-48ec-4e39-93ac-ffcbaf10fc0f
relation.isAuthorOfPublicatione3f02ab4-c9cc-4bdf-8476-6afab22c880c
relation.isAuthorOfPublication.latestForDiscovery81da925b-fa46-4a1a-96fd-ce35568fa423
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