Publication: Immunolocalization of histamine H3 receptors on endocrine cells in the rat gastrointestinal tract
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Date
2008
Authors
Grandi, Daniela ; Shenton, Fiona C. ; Chazot, Paul L. ; Morini, Giuseppina
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The histamine H3 receptor (H3R) has been
identified in the gastrointestinal tract of the rat by
immunohistochemistry, using the first validated anti-H3
receptor antibody. Immunoreactivity to H3R was
exclusively localized to the endocrine cells scattered in
the gastrointestinal mucosa, with positive cells being
prominently abundant in the gastric fundus, while they
were rarely found in the other regions. In the fundus,
positive cells were distributed in the lower half of the
mucosa and their number significantly decreased after a
24 h-fasting period. Double-labeling studies were
undertaken to identify the H3R-immunoreactive cell
types in the fundic and antral mucosa. The H3Rimmunoreactive
cells were positive for chromogranin A.
In the fundus, approximately 90% of cells positive to
H3R were also positive to the histamine-forming
enzyme, histidine decarboxylase. None of the cells
expressing H3R displayed immunoreactivity for gastrin,
somatostatin or ghrelin. Location, the influence of food
deprivation and colocalization with histidine
decarboxylase indicate that H3R positive cells
correspond to the enterochromaffin-like cells (ECL).
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