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Brassica oleracea L. var. italica Aquaporin reconstituted proteoliposomes as nanosystems for resveratrol encapsulation

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dc.contributor.authorYepes Molina, Lucía
dc.contributor.authorTeruel Puche, José Antonio
dc.contributor.authorJohanson, Urban
dc.contributor.authorCarvajal, Micaela
dc.contributor.departmentBioquímica y Biología Molecular A
dc.date.accessioned2026-01-15T08:43:27Z
dc.date.available2026-01-15T08:43:27Z
dc.date.copyright© 2024 by the authors
dc.date.issued2024-02-06
dc.description.abstractAquaporins (AQPs), membrane proteins responsible for facilitating water transport, found in plant membrane vesicles (MV), have been related to the functionality and stability of MV. We focused on AQPs obtained from broccoli, as they show potential for biotechnological applications. To gain further insight into the role of AQPs in MV, we describe the heterologous overexpression of two broccoli AQPs (BoPIP1;2 and BoPIP2;2) in Pichia pastoris, resulting in their purification with high yield (0.14 and 0.99 mg per gram cells for BoPIP1;2 and BoPIP2;2). We reconstituted AQPs in liposomes to study their functionality, and the size of proteoliposomes did not change concerning liposomes. BoPIP2;2 facilitated water transport, which was preserved for seven days at 4 °C and at room temperature but not at 37 °C. BoPIP2;2 was incorporated into liposomes to encapsulate a resveratrol extract, resulting in increased entrapment efficiency (EE) compared to conventional liposomes. Molecular docking was utilized to identify binding sites in PIP2s for resveratrol, highlighting the role of aquaporins in the improved EE. Moreover, interactions between plant AQP and human integrin were shown, which may increase internalization by the human target cells. Our results suggest AQP-based alternative encapsulation systems can be used in specifically targeted biotechnological applications.
dc.formatapplication/pdf
dc.format.extent15
dc.identifier.citationInternational Journal of Molecular Sciences, 2024, Vol 24, N. 5 : 1987
dc.identifier.doihttps://doi.org/10.3390/ijms25041987
dc.identifier.eissn1422-0067
dc.identifier.issn1661-6596
dc.identifier.urihttp://hdl.handle.net/10201/186971
dc.languageeng
dc.publisherMDPI
dc.relationThis study formed part of the AGROALNEXT program and was supported by the Spanish Ministerio de Ciencia e Innovación with funding from the European Union NextGenerationEU (PRTR-C17.I1) and by Fundación Seneca with funding from the Comunidad Autónoma Región de Murcia (CARM). L. Yepes-Molina was funded by a PhD student grant (FPU17/02261) and a complementary mobility grant (EST18/00178). Formas 2004-1952, Olle Engkvists stiftelse 194-0704 and the Crafoord foundation 20190841: U.J.
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/25/4/1987
dc.rightsAttribution 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectBroccoli
dc.subjectMembrane transporter reconstitution
dc.subjectProteoliposomes
dc.subjectNanocarrier
dc.subjectAquaporin
dc.subject.odsObjetivo 6: Agua y saneamiento
dc.titleBrassica oleracea L. var. italica Aquaporin reconstituted proteoliposomes as nanosystems for resveratrol encapsulation
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublicationes
relation.isAuthorOfPublicationbd0ae640-4c40-439e-bf79-a87fea6c8898
relation.isAuthorOfPublication.latestForDiscoverybd0ae640-4c40-439e-bf79-a87fea6c8898
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