Publication: Physicochemical, cytotoxicity and in vivo biocompatibility of a highplasticity calcium-silicate based material
Authors
Rodríguez Lozano, Francisco Javier ; Oñate Sánchez, Ricardo E. ; Ferreira, Cláudio M.A. ; Sassone, Luciana M. ; Gonçalves, Alexia S. ; Carvalho, Jorge José de ; Tomás-Catalá, Christopher J. ; Nogueira Leal Silva, Emmanuel João ; García Bernal, David
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info:eu-repo/semantics/article
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Open Access This article is licensed under a Creative Commons Attribution 4.0 International
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© Te Author(s) 2019
Abstract
The purpose of this work was to evaluate the physicochemical properties, the cytotoxicity and in
vivo biocompatibility of MTA Repair HP (MTA HP) and White MTA (WMTA). The setting time, fow,
radiopacity and water solubility were assessed. To the cytotoxicity assay, primary human osteoblast
cells were exposed to several dilutions of both materials eluates. MTT assay, apoptosis assay and
cell adhesion assay were performed. The in vivo biocompatibility was evaluated through histological
analysis using diferent staining techniques. No diferences were observed between MTA HP and WMTA
for setting time, radiopacity, solubility and water absorption (P >0.05). However, MTA HP showed a
signifcantly higher fow when compared to WMTA (P<0.05). Cell viability results revealed that the
extracts of WMTA and MTA HP promoted the viability of osteoblasts. After incubation of cells with
the endodontic cement extracts, the percentage of apoptotic or necrotic cells was very low (<3%).
Furthermore, SEM results showed a high degree of cell proliferation and adhesion on both groups. MTA
HP showed similar in vivo biocompatibility to the WMTA and the control group in all time-points. The
MTA HP presented adequate physicochemical and biological properties with improved fow ability when
compared to WMTA. Such improved fow ability may be a result of the addition of a plasticizing agent
and should be related to an improvement in the handling of MTA HP.
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