Publication: Daily rhythms of the expression of genes from the somatotropic axis : The influence on tilapia (Oreochromis niloticus) of feeding and growth hormone administration at different times
Authors
Costa, Leandro ; Rosa, Priscila ; Fortes Silva, Rodrigo ; Sánchez Vázquez, Francisco Javier
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Publisher
Elsevier
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DOI
https://doi.org/10.1016/j.cbpc.2015.12.008
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info:eu-repo/semantics/article
Description
©<2016>. This manuscript version is made available under the CC-BY-NC-ND license http://creativecommons.org/licenses/ccbyncdd/4.0/
This document is the acepted version of a Published Work that appeared in final form in [Comparative Biochemistry and Physiology - Part C:].To accessthe final edited and published work see [https://doi.org/10.1016/j.cbpc.2015.12.008]
Abstract
The aim of this research was to investigate the presence of daily rhythms in the
somatotropic axis of tilapia fed at two times (mid-light, ML or mid-dark, MD) and the
influence of the time of day of growth hormone (GH) administration on the response of
this axis. Two different GH injection times were tested: ZT 3 (3 h after lights on) and
ZT 15 (3 h after lights off). In both experiments, the mRNA expression levels of
hypothalamic pituitary adenylate cyclase-activating polypeptide (pacap), pituitary
growth hormone (gh), liver insulin-like growth factors (igf1 and igf2a), and liver and
muscle growth hormone receptors (ghr1 and ghr2) and IGF receptors (igf1ra and igf2r)
were evaluated by means of qPCR. Daily rhythms were observed in the liver for ghr1,
ghr2 and igf2r but only in fish fed at ML, with the acrophases located in the light phase
(ZT 3:30, 3:31 and 7:38 h, respectively). In the muscle, ghr1 displayed a significant
rhythm in both groups and ghr2 in ML fed fish (acrophases at ZT 5:29, 7:14 and 9:23
h). The time of both GH administration and feeding influenced the response to GH
injection: ML fed fish injected with GH at ZT 15 h showed a significant increase in
liver igf1, igf2a and ghr2; and muscle ghr2 expression. This is the first report that
describes the existence of daily rhythms in the somatotropic axis of tilapia and its time-
dependent responses of GH administration. Our results should be considered when
investigating the elements of the somatotropic axis in tilapia and GH administration.
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Citation
Comparative Biochemistry and Physiology Part C, Volumes 181–182, 2016,pp: 27-34
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