Digestive glands extraction and precise pigment analysis support the exclusion of the carnivorous plant Dionaea muscipula Ellis from the Caryophyllales order

Henarejos Escudero, Paula; Guadarrama-Flores, Berenice; Gandía Herrero, Fernando; García Carmona, Francisco

Publication:
Digestive glands extraction and precise pigment analysis support the exclusion of the carnivorous plant Dionaea muscipula Ellis from the Caryophyllales order

dc.contributor.author	Henarejos Escudero, Paula
dc.contributor.author	Guadarrama-Flores, Berenice
dc.contributor.author	Gandía Herrero, Fernando
dc.contributor.author	García Carmona, Francisco
dc.contributor.department	Bioquímica y Biología Molecular A	es
dc.date.accessioned	2021-02-11T12:45:18Z
dc.date.available	2021-02-11T12:45:18Z
dc.date.issued	2018-09
dc.description.abstract	In the order Caryophyllales, plants synthesize betalains instead of anthocyanins, with only two exceptions, the Caryophyllaceae and Molluginaceae. Dionaea muscipula Ellis was included in the Caryophyllales order but recent research based on genetic studies proposed the consideration of the Droseraceae family into the Nepenthales order. In this work we face the dilemma of the phylogenetic classification of Dionaea from a phytochemical point of view. Dionaea’s pigments were analyzed by using techniques of structural analysis. Extracts from the leaves, mature stem and flowers of different specimens of Dionaea were analyzed, to find possible differences in the types of pigments or in their proportion in different parts of the plant. These extracts were analyzed by spectrophotometry, HPLC co-elution and ESI-MS/MS. In addition, digestive glands were extracted from the snap trap with minor sample manipulation and by reducing the non-pigmented plant tissue. Considering only the digestive glands instead of whole snap traps, the analyses allowed to quantitate and elucidate the structure of the compounds responsible for the red coloration: delphinidin-3-O-glucoside (myrtillin), cyanidin-3-O-glucoside (kuromanin) and a third compound, the aglycone cyanidin, detected in the species for the first time. The unambiguous results of the present work support the exclusion of Dionaea from the Caryophyllales.	es
dc.format	application/pdf	es
dc.format.extent	27	es
dc.identifier.citation	Plant Science,2018, 274, 342–348
dc.identifier.doi	https://doi.org/10.1016/j.plantsci.2018.06.013
dc.identifier.issn	0143-7208
dc.identifier.uri	http://hdl.handle.net/10201/103087
dc.language	eng	es
dc.relation	TÍTULO DEL PROYECTO: "Produccion biológica de los pigmentos antioxidantes betalaínas y evaluación de su capacidad funcional en modelo in vivo" AGL2014-57431 ENTIDAD FINANCIADORA: Ministerio de Economía y Competitividad TÍTULO DEL PROYECTO: "Protección frente a factores ambientales relacionados con el estrés oxidativo mediada por alimentos con componentes bioactivos hidrofílicos e hidrofóbicos en modelo in vivo" AGL2017-86526 ENTIDAD FINANCIADORA: Ministerio de Economía y Competitividad TÍTULO DEL PROYECTO: "Biotechnological design of novel health-promoting compounds: enzymatic production, molecular stabilization and testing" 19893/GERM/15 . ENTIDAD FINANCIADORA: Fundación Séneca, Agencia de Ciencia y Tecnología de la Región de Murcia. " Programa de Ayudas a Grupos de Excelencia de la Región de Murcia" (Plan Regional de Ciencia y Tecnología)	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Atribución-NoComercial-SinDerivadas 3.0 España	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/es/	*
dc.subject	Anthocyanins	es
dc.subject	betalains	es
dc.subject	bioactive	es
dc.subject	Dionaea muscipula Ellis	es
dc.subject	red pigment	es
dc.subject	digestive gland	es
dc.subject.other	CDU::5 - Ciencias puras y naturales	es
dc.title	Digestive glands extraction and precise pigment analysis support the exclusion of the carnivorous plant Dionaea muscipula Ellis from the Caryophyllales order	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
relation.isAuthorOfPublication	db7a1a52-fec7-48eb-91e0-abe5e715b483
relation.isAuthorOfPublication	77e02ea7-d1b7-4992-bc4a-c6e8c0cce1a8
relation.isAuthorOfPublication.latestForDiscovery	db7a1a52-fec7-48eb-91e0-abe5e715b483