Publication: Dipeptidyl peptidase IV (DPPIV) activity in the tear fluid as an indicator of the severity of
corneal injury: a histochemical and biochemical study
Authors
Cejkova, J. ; Zvárová, Z. ; Cejka, C.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Comparative histochemical and biochemical
studies on the catalytically active protease Dipeptidyl
peptidase IV (DPPIV), have been performed in the
rabbit cornea and the tear fluid using a sensitive
fluorogenic substrate, Gly-Pro-7-amino-4-
Trifluoromethyl Coumarine (AFC). In both normal and
experimentally injured corneas, DPPIV activity was
detected histochemically and in the tear fluid
biochemically. In contrast to the normal cornea where
DPPIV activity was absent and in the tear fluid where it
was low, during continuous wearing of contact lenses or
repeated irradiation of the cornea with UVB rays, slight
DPPIV activity appeared first in the superficial layers of
the corneal epithelium, while later increased activity was
present in the whole epithelium. This paralleled elevated
DPPIV activity in the tear fluid. Moreover, during
continuous contact lens wear, the increased DPPIV
activity in the tear fluid was, in many cases, coincidental
with the presence of capillaries in the limbal part of the
corneal stroma. After severe alkali burns when corneal
ulcers appeared, collagen fragments were active for
DPPIV, which was associated with high DPPIV activity
in the tear fluid. In conclusion, Gly-Pro-AFC was found to be useful for comparative histochemical and
biochemical studies on DPPIV activity in the
experimentally injured rabbit eye. Using the method of
the tear film collection by a short touch of substrate
punches to the respective site of the cornea or
conjunctiva we can show that in experimental injuries
(wearing of contact lenses, irradiation of the cornea with
UVB rays), the damaged corneal cells were the main
source for DPPIV activity in the tear fluid. It is
suggested that the activity of DPPIV measured in the tear fluid might serve as an indicator of early corneal
disorders, e.g. corneal vascularization related to contact
lens wear.
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