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Oral and Parenteral Vaccination against Escherichia coli in Piglets Results in Different Responses

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dc.contributor.authorRamis Vidal, Manuel Guillermo
dc.contributor.authorPérez-Esteruelas, Lorena
dc.contributor.authorGómez-Cabrera, Carolina G.
dc.contributor.authorDe Pascual-Monreal, Clara
dc.contributor.authorGonzalez-Guijarro, Belén
dc.contributor.authorPárraga Ros, Ester
dc.contributor.authorSánchez-Uribe, Pedro
dc.contributor.authorClaver-Mateos, Miguel
dc.contributor.authorMendonça-Pascoal, Livia
dc.contributor.authorMartínez-Alarcón, Laura
dc.contributor.departmentAnatomía y Anatomía Patológica Comparada
dc.date.accessioned2025-12-23T13:59:28Z
dc.date.available2025-12-23T13:59:28Z
dc.date.copyright© 2022 by the authors
dc.date.issued2022-10-22
dc.description.abstractThe available E. coli vaccines involve two main types (inactivated and live non-pathogenic) and two routes of administration (oral and parenteral) but the mechanism by which both vaccines and routes of administration work is not yet fully elucidated. The influence of a parenteral vaccine (PV) and an oral one (OV) was studied by analyzing the gene expression of biomarkers indicating cellular infiltration (calprotectin, CAL), tight junction proteins (occludin OCL, and zonulin ZON) that maintain intestinal paracellular integration and two proinflammatory (IFN- ) and anti-inflammatory (TGF- ) mediator cytokines, as well as histomorphology and IgA production cell density. Differences were observed in CAL, more infiltrated in orally vaccinated animals; OCL also increased in orally vaccinated animals, and higher density of IgA-producing cells in ileum for orally vaccinated groups. Cytokine expression is also different; and there is a lower mRNA for IFN- in the parenteral than in the oral vaccinated animals. Finally, the villus height-to-crypt depth ratio was higher in the orally vaccinated groups. The data collectively show clear and different effects derived from the use of each type of vaccine, route of administration and regimen. The results suggest a more rapid and direct effect of oral vaccination and a state of suppression in the absence of a second oral stimulus by the pathogen.
dc.formatapplication/pdf
dc.format.extent17
dc.identifier.citationAnimals 2022, 12, 2758.
dc.identifier.doihttps://doi.org/10.3390/ani12202758
dc.identifier.eissn2076-2615
dc.identifier.urihttp://hdl.handle.net/10201/182349
dc.languageeng
dc.publisherMDPI
dc.relationThis trial was funded by ELANCO ANIMAL HEALTH.
dc.relation.publisherversionhttps://www.mdpi.com/2076-2615/12/20/2758
dc.rightsAttribution 4.0 International
dc.rightsAttribution 4.0 International
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectEscherichia coli
dc.subjectVaccine
dc.subjectOral pathway
dc.subjectParenteral pathway
dc.subjectBiomarkers
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleOral and Parenteral Vaccination against Escherichia coli in Piglets Results in Different Responses
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublicationes
relation.isAuthorOfPublicationb5604a5c-a21e-4ebf-b707-eedcf30b0dde
relation.isAuthorOfPublication269b9699-1b31-4bb4-82ab-517084bcf737
relation.isAuthorOfPublication.latestForDiscoveryb5604a5c-a21e-4ebf-b707-eedcf30b0dde
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