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Variation of human salivary alpha-amylase proteoforms in three stimulation models

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dc.contributor.authorContreras Aguilar, María Dolores
dc.contributor.authorVialaret, Jerome
dc.contributor.authorDeville de Périère, Dominique
dc.contributor.authorEscribano Tortosa, Damián
dc.contributor.authorLehmann, Sylvain
dc.contributor.authorTecles Vicente, Fernando
dc.contributor.authorCerón Madrigal, José Joaquín
dc.contributor.authorHirtz, Christophe
dc.contributor.departmentMedicina y Cirugía Animal
dc.date.accessioned2024-01-31T08:44:08Z
dc.date.available2024-01-31T08:44:08Z
dc.date.issued2020-01
dc.description©2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted version of a Published Work that appeared in final form in Clinical Oral Investigations. To access the final edited and published work see https://doi.org/10.1007/s00784-019-03021-9
dc.description.abstractObjectives To evaluate the sAA proteoforms’ expression during different stimulation situations. Materials and methods This study evaluated the salivary alpha-amylase (sAA) proteoforms’ behavior by western blot (WB) analysis and high-resolution mass spectrometry (LC-MS/MS) in different situations that produce increases in sAA activity. For this purpose, six healthy women with a similar body mass index, age, and fit, underwent different sAA stimulation tests, such as acetic acid stimulation, psychological stress using the standardized Trier social stress test, and physical effort using the Cooper treadmill test. Results The three models showed an increase in sAA activity. The WB demonstrated seven common bands observed in the six women (band one at 59 kDa, two at 56 kDa, three at 48 kDa, four at 45 kDa, five at 41 kDa, six at 36 kDa, and seven at 14 kDa), in which sAA protein was identified. The individual WB analysis showed that band two, which corresponded to the native non- glycosylated sAA proteoform, had a higher increase after the three sAA stimulation inducers, and this band was also the only proteoform correlated with sAA activity (r = 0.56, P= 0.001). In addition, when the label-free quantification analysis was performed, the different proteoforms showed different responses depending on the type of stimulation. Conclusions This preliminary study showed that the diverse sAA proteoforms’ expression depends on the different stimulation models. Clinical relevance This study opens new perspectives and challenges for the use of the different alpha-amylase proteoforms as possible biomarkers in addition to the sAA activity.es
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dc.format.extent12es
dc.identifier.citationClinical Oral Investigations, 24(1), 475-486
dc.identifier.doihttps://doi.org/10.1007/s00784-019-03021-9
dc.identifier.issn1436-3771
dc.identifier.issn1432-6981
dc.identifier.urihttp://hdl.handle.net/10201/138247
dc.languageenges
dc.relationÁmbito Regional, Fundación Séneca, Convocatoria de Ayudas a las Unidades y Grupos de Excelencia Científica de la Región de Murcia (Programa Séneca 2014).es
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s00784-019-03021-9es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectSalivary alpha-amylasees
dc.subjectProteoformses
dc.subjectInter-individual variabilityes
dc.subjectStresses
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología::616.8 - Neurología. Neuropatología. Sistema nerviosoes
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología::616.3 - Patología del aparato digestivo. Odontologíaes
dc.titleVariation of human salivary alpha-amylase proteoforms in three stimulation modelses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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