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Accelerated tubular cell senescence in SMP30 knockout mice

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dc.contributor.authorYumura, W.es
dc.contributor.authorImasawa, T.es
dc.contributor.authorSuganuma, S.
dc.contributor.authorIshigami, A.
dc.contributor.authorHanda, S.
dc.contributor.authorKubo, S.
dc.contributor.authorJoh, K.
dc.contributor.authorMaruyama, N.
dc.date.accessioned2011-06-30T12:01:40Z
dc.date.available2011-06-30T12:01:40Z
dc.date.issued2006
dc.description.abstractAn experimental model with accelerated but not drastic renal senescence seemed useful to recognize the mechanisms of how kidney function deteriorates with age. Senescence marker protein-30 (SMP30), whose expression decreased with age and was sexindependent, is mainly expressed in hepatocytes and proximal tubular cells. Therefore, we established a SMP30 deficient strain of mice with a C57BL/6 background by gene targeting to investigate whether this molecule is involved in renal tubular cell senescence. Male SMP30 knockout (SMP30Y/-) mice and male wild-type (SMPY/+) mice (n=5) aged 12 months were examined histologically. Their tubular epithelia showed the deposition of lipofuscin and the presence of senescence-associated ß-galactosidase (SA-ß-GAL). However, no tubular cells were atrophic. In electron microscopy, SMP30-KO mice showed markedly enlarged lysosomes containing an electron dense substance. These are convincing hallmarks of senescence. We recognized the early manifestation of senescence hallmarks in SMP30-KO mice at 12 months old. Thus, this model represents the first report of a mouse strain that manifests accelerated ordinal senescence in a kidney after gene manipulation.es
dc.formatapplication/pdfes
dc.format.extent6es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/22581
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectRenal senescencees
dc.subjectTubular cellses
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleAccelerated tubular cell senescence in SMP30 knockout micees
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.21, nº11 (2006)