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Early acquisition of bowel segment-specific Bcl-2 homolog expression profiles during development of the human ileum and colon

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dc.contributor.authorVachon, P.H.es
dc.contributor.authorCardin, E.es
dc.contributor.authorHarnois, C.
dc.contributor.authorReed, J.C.
dc.contributor.authorPlourde, A.
dc.contributor.authorVézina, A.
dc.date.accessioned2011-05-11T10:55:58Z
dc.date.available2011-05-11T10:55:58Z
dc.date.issued2001
dc.description.abstractThe adult small and large intestines display distinct expression profiles of Bcl-2 homologs, known regulators of apoptosis. This is thought to indicate that control mechanisms of intestinal apoptosis are gut segment-specific. Little is known on the expression of Bcl-2 homologs during gut development. In man, intestinal features and functions are acquired largely by mid-gestation (18-20 wks); the question whether segment-specific controls of intestinal apoptosis are also acquired early during development remains open. In the present study, we approached this by investigating the expression of six Bcl-2 homologs (Bcl-2, Bcl-XL, Mcl- 1, Bax, Bak, Bad), and one nonhomologous associated molecule (Bag-1), during development of the human ileum and colon (12-20 wks of gestation). Beginning at 18 wks, we found that the epithelial localization of Bcl-2 homologs displayed differential patterns (or gradients) in both the ileum and colon; however, the patterns of some of the homologs differed between the two segrnents. For instance, Bag-1 and Bcl-2 exhibited crypt-villus decreasing gradients of expression in the ileum but not in the colon, whereas Mcl-1 displayed differing compartimentalizations between the two segments. Further analyses indicated that the steady-state expression levels of Bcl-2 homologs underwent modulations between 12 and 20 wks; however, the obsewed developmental profiles contrasted significantly between the two segments. For example, Bcl-2, Bag-1 and Bak levels increased in the colon, but the levels of these same homo.logs decreased in the ileum. Furthermore, by 18-20 wks, we found that the expression levels of each Bcl-2 homolog analyzed differed greatly between the ileum and colon. Altogether, these data indicate that the expression of Bcl-2 homologs is modulated differentially during human gut development in order to establish, by midgestation, distinct expression profiles for the small and large intestines. This in turn suggests that gut segmentspecific control mechanisms of human intestinal apoptosis are acquired early during fetal life.es
dc.formatapplication/pdfes
dc.format.extent14es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/20660
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectGutes
dc.subjectEnterocytees
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología::616.3 - Patología del aparato digestivo. Odontologíaes
dc.titleEarly acquisition of bowel segment-specific Bcl-2 homolog expression profiles during development of the human ileum and colones
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.16, nº 2 (2001)