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Esterase activity (EA), total oxidant status (TOS) and total antioxidant capacity (TAC) in gills of Mytilus galloprovincialis exposed to pollutants: analytical validation and effects evaluation by single and mixed heavy metal exposure

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dc.contributor.authorFranco, Lorena
dc.contributor.authorRomero García, Diego
dc.contributor.authorGarcía-Navarro, José A.
dc.contributor.authorTeles, Mariana
dc.contributor.authorTvarijonaviciute, Asta
dc.contributor.departmentCiencias Sociosanitarias
dc.date.accessioned2024-03-11T11:51:35Z
dc.date.available2024-03-11T11:51:35Z
dc.date.issued2015-12-17
dc.description©2015 Elsevier. This document is the Published version of a Published Work that appeared in final form in Marine Pollution Bulletin. To access the final edited and published work see https://doi.org/10.1016/j.marpolbul.2015.12.010es
dc.description.abstractThe aims of the present study were to optimize and validate methods for esterase activity (EA), total oxidant status (TOS) and total antioxidant capacity (TAC) determination in mussel' gills, and to establish the relationships between these biomarkers and Pb, Cd and Cu pollution, in single form and ternary mixture. Two different buffers for sample homogenization, the need of ultracentrifugation, and analytical validation were evaluated. Coefficients of variation, when buffer without additives and ultracentrifugation were used, were b15%, and recovery were 97%–109% in all cases. The EA response tends to decrease with treatments, TOS decreased significantly in Cd and ternary groups, while TAC tended to increase in treatments with Pb, Cd and ternary groups. In conclusion, the methods for EA, TOS and TAC measurements in gills of mussel were precise and accurate and could be interesting resources in biomonitoring programmes.es
dc.formatapplication/pdfes
dc.format.extent6es
dc.identifier.citationMarine Pollution Bulletin, Vol. 102, N. 1, 2016, pp. 30-35
dc.identifier.doihttps://doi.org/10.1016/j.marpolbul.2015.12.010
dc.identifier.issnPrint: 0025-326X
dc.identifier.issnElectronic: 1879-3363
dc.identifier.urihttp://hdl.handle.net/10201/140087
dc.languageenges
dc.publisherElsevieres
dc.relationFinancial support was provided by the Program “Juan de la Cierva” of ‘Ministerio de Economia y Competitividad’, Spain, through a postdoctoral grant.es
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0025326X15302162?via%3Dihubes
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectLeades
dc.subjectCadmiumes
dc.subjectCopperes
dc.subjectTernary mixturees
dc.subjectOxidative stresses
dc.subjectMussellses
dc.subjectMusselses
dc.titleEsterase activity (EA), total oxidant status (TOS) and total antioxidant capacity (TAC) in gills of Mytilus galloprovincialis exposed to pollutants: analytical validation and effects evaluation by single and mixed heavy metal exposurees
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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