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Ghrelin and adipose-derived mesenchymal stromal cells improve nerve regeneration in a rat model of epsilon-caprolactone conduit reconstruction

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dc.contributor.authorHernández Cortés, Pedro
dc.contributor.authorToledo Romero, Miguel Angel
dc.contributor.authorDelgado, Mario
dc.contributor.authorGonzalez Rey, Elena
dc.contributor.authorGómez Sánchez, Rafael
dc.contributor.authorPrados Olleta, Nicolás
dc.contributor.authorAneiros Fernández, José
dc.contributor.authorCrespo Lora, Vicente
dc.contributor.authorAguilar, Mariano
dc.contributor.authorGalindo Moreno, Pablo
dc.contributor.authorO’Valle, Francisco
dc.date.accessioned2022-02-23T12:23:06Z
dc.date.available2022-02-23T12:23:06Z
dc.date.issued2017
dc.description.abstractObjective. Attempts have been made to improve nerve conduits in peripheral nerve reconstruction. We investigated the potential therapeutic effect of adipose-derived mesenchymal cells (ASCs) and ghrelin (GHR), a neuropeptide with neuroprotective, trophic, and developmental regulatory actions, on peripheral nerve regeneration in a model of severe nerve injury repaired with nerve conduits. Material and methods. The right sciatic nerves of 24 male Wistar rats were 10-mm transected unilaterally and repaired with Dl-lactic-ε-caprolactone conduits. Rats were then treated locally with saline, ASCs, or GHR. At 12 weeks post-surgery, we assessed limb function by measuring ankle stance angle and percentage muscle mass reduction and evaluated the histopathology, immunohistochemistry, ultrastructure, and morphometry of myelinated fibers. Main Results. Rats receiving GHR or ASCs showed no significant increased functional recovery in ankle stance angle (p=0.372) but a higher nerve area (p=0.015), myelin area (p=0.046) and number of myelinated fibers (p=0.012) in the middle and distal segments of operated sciatic nerves in comparison to saline-treated control animals. Conclusion. These results suggest that utilization of ghrelin or ASCs may improve nerve regeneration using Dl-lactic-ε-caprolactone conduits.es
dc.formatapplication/pdfes
dc.format.extent11es
dc.identifier.citationHistology and Histopathology, Vol.32, nº6, (2017)
dc.identifier.doiDOI: 10.14670/HH-11-828
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/117324
dc.languageenges
dc.publisherUniversidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectEpsilon caprolactonees
dc.subjectGhrelines
dc.subjectPeripheral nerve injuryes
dc.subjectNerve regenerationes
dc.subjectRates
dc.subjectSciatic nervees
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleGhrelin and adipose-derived mesenchymal stromal cells improve nerve regeneration in a rat model of epsilon-caprolactone conduit reconstructiones
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.32, nº6 (2017)