Publication: A quantitative investigation of microvascular changes in the thyroid gland after infrared ,IR, laser radiation
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Date
1999
Authors
Parrado, C. ; Carrillo de Albornoz, F. ; Vidal, L. ; Perez de Vargas, I.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
We present an ultrastructural study of thyroid
capillaries in which 50-day-old rats Wistar rats, were
irradiated with an infrared (IR) laser, (total dose, 46.80
~ / c m ~th)e, tissue quantified 1 day after ending treatment
and a quantitative capillary analysis carried out by light
and electron microscopy. Light microscopy was used to
calculate capillary volume density revealing a significant
increase in the irradiated rats. The quantitative
measurement of parameters by electron microscopy
required a two stage analysis: Level I, Electron
Microscopy (Magnification ~ 5 , 0 0 0 ) ;a nd Level 11,
Electron Microscopy (Magnification ~26,000)A. t Level
I, the following parameters were measured in each
capillary: capillary area, capillary diameter, luminal area,
luminal diameter, endothelial area, nuclear area and
mean endothelial thickness. At Level 11, pinocytotic
vesicle diameter and their numerical density in
endothelial cells were evaluated. Electron microscopic
analysis revealed an increased luminal area in the
capillaries of the irradiated rats. They also presented a
decrease in endothelial cell thickness and vesicular
diameter and an increase in vesicle numerical density.
This latter increase is indicative of presumptive changes
in capillary permeability, but the possible functional
significance of these morphological changes in the
endothelial cells requires further investigation.
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