Dietary fatty acids affect mitochondrial phospholipid compositions and mitochondrial gene expression of rainbow trout liver at different ages

Almaida Pagán, Pedro Francisco; De Santis, C; Rubio-Mejia, O L; Tocher, D R

Publication:
Dietary fatty acids affect mitochondrial phospholipid compositions and mitochondrial gene expression of rainbow trout liver at different ages

dc.contributor.author	Almaida Pagán, Pedro Francisco
dc.contributor.author	De Santis, C
dc.contributor.author	Rubio-Mejia, O L
dc.contributor.author	Tocher, D R
dc.contributor.department	Fisiología
dc.date.accessioned	2024-06-18T11:33:35Z
dc.date.available	2024-06-18T11:33:35Z
dc.date.issued	2014-11-15
dc.description	© Springer-Verlag Berlin Heidelberg 2014. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted version of a Published Work that appeared in final form in Journal of Comparative Physiology B: Biochemical, Systems, and Environmental Physiology. To access the final edited and published work see https://doi.org/10.1007/s00360-014-0870-8
dc.description.abstract	Mitochondria are among the first responders to various stressors that challenge the homeostasis of cells and organisms. Mitochondrial decay is generally associated with impairment in the organelle bioenergetics function and increased oxidative stress, and it appears that deterioration of mitochondrial inner membrane phospholipids (PL), particularly cardiolipin (CL), and accumulation of mitochondrial DNA (mtDNA) mutations are among the main mechanisms involved in this process. In the present study, liver mitochondrial membrane PL compositions, lipid peroxidation, and mtDNA gene expression were analyzed in rainbow trout fed three diets with the same base formulation but with lipid supplied either by fish oil (FO), rapeseed oil (RO), or high DHA oil (DHA) during 6 weeks. Specifically, two feeding trials were performed using fish from the same population of two ages (1 and 3 years), and PL class compositions of liver mitochondria, fatty acid composition of individual PL classes, TBARS content, and mtDNA expression were determined. Dietary fatty acid composition strongly affected mitochondrial membrane composition from trout liver but observed changes did not fully reflect the diet, particularly when it contained high DHA. The changes were PL specific, CL being particularly resistant to changes in DHA. Some significant differences observed in expression of mtDNA with diet may suggest long-term dietary effects in mitochondrial gene expression which could affect electron transport chain function. All the changes were influenced by fish age, which could be related to the different growth rates observed between 1- and 3-year-old trout but that could also indicate age-related changes in the ability to maintain structural homeostasis of mitochondrial membranes.	es
dc.format	application/pdf	es
dc.format.extent	14	es
dc.identifier.citation	Journal of Comparative Physiology B, 2015, Vol. 185, pp. 73–86
dc.identifier.doi	https://doi.org/10.1007/s00360-014-0870-8
dc.identifier.issn	Print: 0174-1578
dc.identifier.issn	Electronic: 1432-136X
dc.identifier.uri	http://hdl.handle.net/10201/142402
dc.language	eng	es
dc.publisher	Springer	es
dc.relation	This research and P.F.A.-P. were funded by a Marie Curie Intra-European Fellowship within the 7th Community Framework Programme (PIEFGA-2011-297964, OLDMITO).	es
dc.relation.publisherversion	https://link.springer.com/article/10.1007/s00360-014-0870-8#rightslink	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	Cardiolipin	es
dc.subject	Diet
dc.subject	Fatty acid
dc.subject	Mitochondria
dc.subject	Rainbow trout
dc.subject	Oxidative stress
dc.subject	Phospholipid
dc.title	Dietary fatty acids affect mitochondrial phospholipid compositions and mitochondrial gene expression of rainbow trout liver at different ages	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es