Publication:
Deleted in malignant brain tumor 1 is secreted in the oviduct and involved in the mechanism of fertilization in equine and porcine species

Simple item page
dc.contributor.authorAmbruosi, B.
dc.contributor.authorAccogli, G.
dc.contributor.authorDouet, C.
dc.contributor.authorCanepa, S.
dc.contributor.authorPascal, G.
dc.contributor.authorMonget, P.
dc.contributor.authorHolmskov, U.
dc.contributor.authorMollenhauer, J.
dc.contributor.authorRobbe-Masselot, C.
dc.contributor.authorVidal, O.
dc.contributor.authorDesantis, S.
dc.contributor.authorGoudet, G.
dc.contributor.authorMoros Nicolás, Carla
dc.contributor.departmentBiología Celular e Histología
dc.date.accessioned2024-01-22T16:24:20Z
dc.date.available2024-01-22T16:24:20Z
dc.date.issued2013
dc.description©2013. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted, version of a Published Work that appeared in final form in Reproduction. To access the final edited and published work see https://doi.org/10.1530/REP-13-0007es
dc.description.abstractOviductal environment affects preparation of gametes for fertilization, fertilization itself, and subsequent embryonic development. The aim of this study was to evaluate the effect of oviductal fluid and the possible involvement of deleted in malignant brain tumor 1 (DMBT1) on IVF in porcine and equine species that represent divergent IVF models. We first performed IVF after pre-incubation of oocytes with or without oviductal fluid supplemented or not with antibodies directed against DMBT1. We showed that oviductal fluid induces an increase in the monospermic fertilization rate and that this effect is canceled by the addition of antibodies, in both porcine and equine species. Moreover, pre-incubation of oocytes with recombinant DMBT1 induces an increase in the monospermic fertilization rate in the pig, confirming an involvement of DMBT1 in the fertilization process. The presence of DMBT1 in the oviduct at different stages of the estrus cycle was shown by western blot and confirmed by immunohistochemical analysis of ampulla and isthmus regions. The presence of DMBT1 in cumulus–oocyte complexes was shown by western blot analysis, and the localization of DMBT1 in the zona pellucida and cytoplasm of equine and porcine oocytes was observed using immunofluorescence analysis and confocal microscopy. Moreover, we showed an interaction between DMBT1 and porcine spermatozoa using surface plasmon resonance studies. Finally, a bioinformatic and phylogenetic analysis allowed us to identify the DMBT1 protein as well as a DMBT1-like protein in several mammals. Our results strongly suggest an important role of DMBT1 in the process of fertilization.es
dc.formatapplication/pdfes
dc.format.extent15es
dc.identifier.doihttps://doi.org/10.1530/REP-13-0007
dc.identifier.issn1741-7899
dc.identifier.issn1470-1626
dc.identifier.urihttp://hdl.handle.net/10201/137546
dc.languageenges
dc.relation‘Institut Franc¸ais du Cheval et de l’Equitation’ (IFCE)es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleDeleted in malignant brain tumor 1 is secreted in the oviduct and involved in the mechanism of fertilization in equine and porcine specieses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
relation.isAuthorOfPublication3acee413-7e4c-4025-90cb-5a0d9cb10014
relation.isAuthorOfPublication.latestForDiscovery3acee413-7e4c-4025-90cb-5a0d9cb10014
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Ambruosi_2013_DMBT1_Reproduction_accepted.pdf
Size:
637.02 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.26 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Artículos