Publication: Lectinhistochemistry and ultrastructure of microglial response to monosodium glutamate-mediated neurotoxicity in the arcuate nucleus
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Date
1999
Authors
Pelaez, B. ; Blazquez, J.L. ; Pastor, F.E. ; Sánchez, A. ; Amat, P.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
In this study we describe the most relevant
morphological features of the microglial reaction that
takes place in the arcuate nucleus (AN) after neurotoxic
injury induced by a single subcutaneous injection of
monosodium glutamate (MSG) in neonatal rats. The
time course of the reaction was evaluated by lectinhistochemistry.
Microglial/macrophagic cells were
labelled with the lectin obtained from Lycopersicon
esculentum and with B4 isolectin from Griffonia
simplicifolia. The microglial response was also studied
by ultrastructural observations. 'The histochemical study
revealed the presence of few reactive microglial cells at
6 h post-injection. These cells were intensely stained and
had a globular morphology but contained no neuronal
debris inside them when observed under the electron
microscope. At 12 h post-injection, the number of
microglial cells had increased and, at the same time,
intense phagocytic activity was observed ultrastructurally.
The microglial reaction peaked at 24 and
36 h post-injection, when the number of microglial/
macrophagic cells was maximum, although the
ultrastructural observations showed that at 36 h the
amount of debris ingested by macrophages was decreased with respect to animals sacrificed at 24 h.
Finally, at 4 days after neurotoxic injection the number
and morphology of microglial cells were similar to those
observed in the control rats. The ultrastructural study
also revealed the existence of microglial cell mitosis in
the territory of the AN together with a strong increase in
the number of supraependymal cells resembling
macrophages in the third ventricle during the lesion. Our
data demonstrate that activated microglial cells initially
extend throughout the damaged territory, but from 24-36
h onwards they are especially patent in the ventrolateral
portions of the AN.
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