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Myosin II regulatory light chain phosphorylation and formin availability modulate cytokinesis upon changes in carbohydrate metabolism.

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dc.contributor.authorPrieto Ruiz, Francisco
dc.contributor.authorGómez Gil, Elisa
dc.contributor.authorMartín García, Rebeca
dc.contributor.authorPérez Díaz, Armando J.
dc.contributor.authorVicente Soler, Jero
dc.contributor.authorFranco, Alejandro
dc.contributor.authorSoto, Teresa
dc.contributor.authorPérez, Pilar
dc.contributor.authorMadrid, Marisa
dc.contributor.authorCansado Vizoso, José
dc.contributor.departmentGenética y Microbiología
dc.date.accessioned2025-01-13T09:07:30Z
dc.date.available2025-01-13T09:07:30Z
dc.date.issued2023-03-10
dc.description© 2023, Prieto-Ruiz et al. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the accepted manuscript version of a published work that appeared in final form in eLife. To access the final edited and published work see https://doi.org/10.7554/eLife.83285
dc.description.abstractCytokinesis, which achieves the separation of daughter cells after mitosis completion, relies in animal cells on a contractile actomyosin ring (CAR), made of actin and class II myosins, whose activity is heavily influenced by regulatory light chain (RLC) phosphorylation. However, in simple eukaryotes such as fission yeast Schizosaccharomyces pombe, regulation of CAR dynamics by RLC phosphorylation seems dispensable. We found that redundant phosphorylation at Ser35 of the S. pombe RLC homolog Rlc1 by the p21-activated kinases Pak1 and Pak2, modulates Myosin II Myo2 activity and becomes essential for cytokinesis and cell growth during respiration. Previously, we showed that the Stress Activated Protein Kinase Pathway (SAPK) MAPK Sty1 controls fission yeast CAR integrity by downregulating formin For3 levels (Gomez-Gil et al.,2020). Here we report that reduced availability of formin For3-nucleated actin filaments for the CAR is the main reason for the required control of myosin II contractile activity by RLC phosphorylation during respiration-induced oxidative stress. Hence, recovery of For3 levels with antioxidants bypasses the control of Myosin II function regulated by RLC phosphorylation to allow cytokinesis and cell proliferation during respiration. Therefore, a fine-tuned interplay between Myosin II function by Rlc1 phosphorylation and environmentally controlled actin filament availability is critical for a successful cytokinesis in response to a switch to a respiratory carbohydrate metabolism.es
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dc.format.extent57es
dc.identifier.citationeLife, 2023, Vol. 12 : e83285
dc.identifier.doihttps://doi.org/10.7554/eLife.83285
dc.identifier.urihttp://hdl.handle.net/10201/148281
dc.languageenges
dc.publishereLife Sciences Publications
dc.relationÁmbito del proyecto: Nacional Agencia financiadora: Agencia Estatal de Investigación Convocatoria: Proyectos de I+D+i, modalidad "generación de conocimiento". Nombre del proyecto: Mecanismos de control de la citocinesis modulados por señales ambientales. Código o número del acuerdo de subvención: PID2020-112569GB-I00es
dc.relation.publisherversionhttps://elifesciences.org/articles/83285es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectCytokinesises
dc.subjectRespiration
dc.subjectMyosin II
dc.subjectFormin
dc.subjectp21 activated kinase
dc.subjectFission yeast
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citologíaes
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::579 - Microbiologíaes
dc.titleMyosin II regulatory light chain phosphorylation and formin availability modulate cytokinesis upon changes in carbohydrate metabolism.es
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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