Publication: Distribution of glutamate receptors in the posterodorsal medial amygdala of adult male rats
Authors
Dalpian, Francine ; Brusco, Janaína ; Calcagnotto, Maria Elisa ; Moreira, Jorge E. ; Rasia-Filho, Alberto A.
item.page.secondaryauthor
item.page.director
Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
publication.page.editor
publication.page.department
DOI
10.14670/HH-11-626
item.page.type
info:eu-repo/semantics/article
Description
Abstract
The rat posterodorsal medial amygdala
(MePD) has a remarkable neuronal plasticity and
responds to olfactory/pheromonal stimuli to modulate
emotional and reproductive behaviors. Glutamate is
locally released by incoming sensorial pathways to
establish and enforce synaptic inputs. Here, we
combined DiI dye and immunolabeling procedure under
confocal microscopy to describe the presence and
distribution of glutamate receptors on neurons of the
MePD of adult male rats. Western blot analysis
interrogated binding specificity. Both AMPA (GluA1-4
subunits) and NMDA (GluN1 subunit) receptors were
immunolabeled on cell bodies and along proximal and
distal dendritic shafts. AMPA receptors were mainly
observed on mushroom and stubby/wide spines, whereas
NMDA receptors were found on thin spines.
Colocalization of AMPA and NMDA receptors occurred
in some spines. Filopodium did not show immunolabeled puncta on it. Our results are different from the
distribution of glutamate receptors in the amygdaloid
lateral nucleus, an upstream area involved with
emotional processing, and suggest a region-specific
excitatory transmission at proximal and distal dendritic
branches. Altogether, these data provide new
information for synaptic processing in the MePD likely
related to the modulation of social behavior in rats.
publication.page.subject
Citation
Histology and Histopathology, vol. 30, nº 11, (2015)
item.page.embargo
Ir a Estadísticas
Este ítem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/3.0/es/