Publication: Morphometric analysis of the tumor associated tissue eosinophilia in the oral squamous
cell carcinoma using different staining techniques
Authors
Lorena, S.C.M. ; Dorta, R.G. ; Landman, G. ; Nonogaki, S. ; Oliveira, D.T.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
In a previous study, we found tumorassociated
tissue eosinophilia (TATE) to be a favourable
prognostic indicator for oral squamous cell carcinomas.
Special techniques such as autofluorescence or
immunohistochemistry are reported to be sometimes
necessary to detect the presence of intact and
degranulating eosinophils within the tumors. The aim of
this study was to compare the number of eosinophils
identified routinely with hematoxylin and eosin stain and
by immunohistochemistry in oral squamous cell
carcinomas with TATE. Thirty specimens of oral
squamous cell carcinoma of the tongue, floor of the
mouth, retromolar area and inferior gingiva with TNM
stages II and III were used for histopathological analysis.
Three-micrometer sections were stained with
hematoxylin and eosin and immunohistochemically with
monoclonal anti-human granulocyte-associated antigen
using a standard streptavidin-biotin-peroxidase complex
technique. The number of eosinophils/mm2 in the
invasive front of the tumors was automatically
quantified in a x400 field using an image computer
analyser. Univariate statistical analysis was carried out
using Student’s t test. The computer-assisted morphometric results showed that there was no
statistically significant difference (p>0.05) in the number
of eosinophils/mm2 identified by hematoxylin and eosin
or immunostaining technique in oral squamous cell
carcinomas with TATE. This result suggests that
hematoxilyn and eosin routine stain is a useful technique
for measuring eosinophils in squamous cell carcinoma
with eosinophilic tumor infiltration.
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