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Specific and non-overlapping functions of testosterone and 11-ketotestosterone in the regulation of professional phagocyte responses in the teleost fish gilthead seabream

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dc.contributor.authorÁguila Martínez, Sonia
dc.contributor.authorCastillo Briceño, P.
dc.contributor.authorSánchez, M.
dc.contributor.authorCabas, Isabel
dc.contributor.authorGarcía Alcázar, Alicia
dc.contributor.authorMeseguer, José
dc.contributor.authorMulero Méndez, Victoriano Francisco
dc.contributor.authorGarcía Ayala, Alfonsa
dc.contributor.departmentBiología Celular e Histología;
dc.contributor.departmentMedicina
dc.date.accessioned2026-02-28T20:55:47Z
dc.date.available2026-02-28T20:55:47Z
dc.date.copyright© 2012 Elsevier Ltd.
dc.date.issued2012-09-06
dc.description.abstractSex hormones, both estrogens and androgens, have a strong impact on immunity in mammals. In fish, the role of androgens in immunity has received little attention and contradictory conclusions have been obtained. However, it is well known that sex steroids are involved in fish growth, osmoregulation and gonad remodelation. In this study, we examine the in vitro effects of testosterone and 11-ketotestosterone, the two main fish androgens, on the professional phagocytes of the teleost fish gilthead seabream (Sparus aurata L.). Although both testosterone and 11-ketotestosterone failed to modulate the respiratory burst of seabream phagocytes, testosterone but not 11-ketotestosterone was able to increase the phagocytic ability of non-activated phagocytes. Curiously, 11-ketotestosterone was more powerful than testosterone at inducing the expression of its own receptor, namely androgen receptor b (ARb), in acidophilic granulocytes (AGs), but none of them affected the basal ARb expression levels in macrophages (MØ). Furthermore, although physiological concentrations of testosterone exerted a pro-inflammatory effect on both AGs and MØs, 11-ketotestosterone showed an anti-inflammatory effect in AGs and a strong pro-inflammatory effect in MØs. Interestingly, both androgens modulated the expression of toll-like receptors in these two immune cell types, suggesting that androgens might regulate the sensitivity of phagocytes to pathogens and damage signals. Testosterone and 11-ketotestosterone have a competitive effect, at least, on the modulation of the expression of some genes. Therefore, our results show for the first time a non-overlapping role for testosterone and 11-ketotestosterone in the regulation of professional phagocyte functions in fish.
dc.formatapplication/pdf
dc.format.extent9
dc.identifier.citationMolecular Immunology, Volume 53, Issue 3, March 2013, Pages 218-226
dc.identifier.doihttps://doi.org/10.1016/j.molimm.2012.08.002
dc.identifier.eissn1872-9142
dc.identifier.issn0161-5890
dc.identifier.urihttp://hdl.handle.net/10201/216461
dc.languageeng
dc.publisherElsevier
dc.relationWe thank the Fundación Séneca, Coordination Center for Research (CARM) for research grant 04538/GERM/06 to A. García-Ayala. We are grateful to the Spanish Ministry of Science for research grant AGL2008-04575-C02-01 and AGL2011-30264-C02-01 to A. García-Ayala. We thank Drs. JL Barja and AE Toranzo for the bacterium, Inma Fuentes for excellent technical assistance and the “Servicio de Apoyo a la Investigación” of the University of Murcia for their assistance with cell culture, flow cytometry and real-time PCR.
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0161589012003616
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectImmunity
dc.subjectTestosterone
dc.subject11-Ketotestosterone
dc.subjectAcidophilic granulocytes
dc.subjectMacrophages
dc.subjectTeleost fish
dc.subject.odsObjetivo 3: Salud
dc.titleSpecific and non-overlapping functions of testosterone and 11-ketotestosterone in the regulation of professional phagocyte responses in the teleost fish gilthead seabream
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublicationes
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relation.isAuthorOfPublication369f9ade-8896-4d91-84c5-e3f94a526070
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relation.isAuthorOfPublication.latestForDiscovery49779dfb-f4b4-4013-9b03-2ad7e3c247b7
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