Publication: Role of hepatic macrophages during the viral haemorrhagic fever induced by African Swine Fever Virus
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Date
2008
Authors
Sánchez-Cordón, Pedro José ; Romero-Trevejo, José Lorenzo ; Pedrera, Mirian ; Sánchez-Vizcaino, José Manuel ; Bautista, María José ; Gómez-Villamandos, José Carlos
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
To ascertain the role played by the various
liver monocyte-macrophage populations in the course of
a viral hemorrhagic fever, fifteen pigs were inoculated
intramuscularly with the highly virulent isolate of
African Swine Fever Virus (ASFV) España-70 and
slaughtered at 1-7 days post-inoculation (dpi). Samples
of liver were fixed in different solutions and routinely
processed for morphological, immunohistochemical and
ultrastructural studies. Viral antigen (vp73) was detected
from 3 dpi onward, mainly in circulating monocytes of
sinusoid and Kupffer’s cells (KC), as well as in portal
macrophages and hepatocytes from 5 dpi. Anti-SWC3
immunolabelled cells were increased from 1 dpi,
peaking between 3 and 5 dpi, thereafter declining until
the end of the experiment. The significant increase in the
number of sinusoidal circulating monocytes and KC
expressing IL-1a, TNFa and IL-6 from 1 dpi, confirmed
the secretory activation of these cells. The results show
that in the course of an ASFV-induced hemorrhagic
syndrome, hepatic macrophage populations undergo
major quantitative and biosynthetic changes prior to
virus detection, suggesting the existence of a mechanism
by which the virus concentrates infectable cells, which
subsequently spread the virus around the body.
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