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Evolutionary conserved pro-inflammatory and antigen presentation functions of zebrafish IFN revealed by transcriptomic and functional analysis

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dc.contributor.authorAzucena López-Munoz
dc.contributor.authorLópez Muñoz, Azucena
dc.contributor.authorSepulcre Cortés, María Pilar
dc.contributor.authorRoca Soler, Francisco José
dc.contributor.authorFigueras, Antonio
dc.contributor.authorMeseguer, José
dc.contributor.authorMulero Méndez, Victoriano Francisco
dc.contributor.departmentBiología Celular e Histología
dc.date.accessioned2026-02-25T16:49:41Z
dc.date.available2026-02-25T16:49:41Z
dc.date.copyright© 2011 Elsevier Ltd.
dc.date.issued2011-02-26
dc.description.abstractIn mammals, IFNγ is the only type II IFN member, whereas most bony fish species have two IFNγ genes, namely IFNγ1 and IFNγ2. We report that both zebrafish IFNγ genes were unable to protect zebrafish larvae against viral infection, despite the fact that they moderately induced the expression of antiviral genes, strongly induced pro-inflammatory and antigen processing and presentation genes, and increased neutrophil numbers. Although both zebrafish IFNγs induced a similar set of immune genes, IFNγ1 was more powerful at inducing pro-inflammatory genes than IFNγ2, which correlated with its ability to promote larval death. Strikingly, IFNγ1-induced larval death was prevented by genetic ablation of the myeloid transcription factor SPI1 but not IL-1β or TNFα, suggesting that professional phagocytes are also one of the main targets of IFNγ in fish. In addition, the usefulness of the zebrafish for the identification of IFNγ-target genes is illustrated by the identification of several genes whose expression is also regulated in murine macrophages by IFNγ, namely two membrane-spanning 4-domain family members and the opioid growth factor receptor. Finally, we found for the first time that the thymic specific proteasome subunit PSMB11/β5t is regulated by IFNγ. Collectively, our data throw light on partially redundant functions of fish IFNγ genes, demonstrate that the pro-inflammatory and antigen presentation functions of IFNγ have been conserved during vertebrate evolution, and highlight the fact that zebrafish is an excellent model for studying IFNγ biology.
dc.formatapplication/pdf
dc.format.extent11
dc.identifier.citationMolecular Immunology 48 (2011) 1073–1083
dc.identifier.doihttps://doi.org/10.1016/j.molimm.2011.01.015
dc.identifier.eissn1872-9142
dc.identifier.issn0161-5890
dc.identifier.urihttp://hdl.handle.net/10201/213682
dc.languageeng
dc.publisherElsevier
dc.relation This work was supported by the Spanish Ministry of Science and Innovation (grants BIO2008-01379 and CSD2007-00002), the Fundación Séneca-Murcia (04538/GERM/06), the Fundación Marcelino Botín, and the University of Murcia (fellowships to A.L.-M.).
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0161589011000381
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectNeutrophils
dc.subjectMacrophages
dc.subjectImmunoproteasome
dc.subjectMyelopoiesis
dc.subjectComparative immunology evolution
dc.subject.odsObjetivo 12: Producción y consumo sostenibles
dc.titleEvolutionary conserved pro-inflammatory and antigen presentation functions of zebrafish IFN revealed by transcriptomic and functional analysis
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublicationes
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relation.isAuthorOfPublication7f46a88a-1ff4-4f80-948e-7d636b80e75d
relation.isAuthorOfPublication369f9ade-8896-4d91-84c5-e3f94a526070
relation.isAuthorOfPublication.latestForDiscovery508910a4-32ba-4c84-8616-ea6c14c4f883
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