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Aptamer-Target-gold nanoparticle conjugates for the quantification of fumonisin B1.

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dc.contributor.authorVicente A Mirón-Mérida
dc.contributor.authorYadira González-Espinosa
dc.contributor.authorYun Yun Gong
dc.contributor.authorYuan Gong
dc.contributor.authorCollado-González, Mar
dc.contributor.authorGoycoolea Valencia, Francisco Martín
dc.contributor.departmentBiología Celular e Histología
dc.contributor.otherFacultades de la UMU
dc.date.accessioned2025-09-25T07:29:54Z
dc.date.available2025-09-25T07:29:54Z
dc.date.copyright© 2021 by the authors
dc.date.issued2021-01-08
dc.description.abstractFumonisin B1 (FB1), a mycotoxin classified as group 2B hazard, is of high importance due to its abundance and occurrence in varied crops. Conventional methods for detection are sensitive and selective; however, they also convey disadvantages such as long assay times, expensive equipment and instrumentation, complex procedures, sample pretreatment and unfeasibility for on-site analysis. Therefore, there is a need for quick, simple and affordable quantification methods. On that note, aptamers (ssDNA) are a good alternative for designing specific and sensitive biosensing techniques. In this work, the assessment of the performance of two aptamers (40 and 96 nt) on the colorimetric quantification of FB1 was determined by conducting an aptamer–target incubation step, followed by the addition of gold nanoparticles (AuNPs) and NaCl. Although MgCl2 and Tris-HCl were, respectively, essential for aptamer 96 and 40 nt, the latter was not specific for FB1. Alternatively, the formation of Aptamer (96 nt)–FB1–AuNP conjugates in MgCl2 exhibited stabilization to NaClinduced aggregation at increasing FB1 concentrations. The application of asymmetric flow field-flow fractionation (AF4) allowed their size separation and characterization by a ultidetection system (UV-VIS, MALS and DLS online), with a reduction in the limit of detection from 0.002 g/mL to 56 fg/mL.
dc.formatapplication/pdf
dc.format.extent18
dc.identifier.citationBiosensors 2021, 11, 18
dc.identifier.doihttps://doi.org/10.3390/bios11010018
dc.identifier.eissn2079-6374
dc.identifier.urihttp://hdl.handle.net/10201/161489
dc.languageeng
dc.publisherMDPI
dc.relationV.A.M.-M. was awarded with a scholarship from CONACYT (Mexico) for completing his PhD studies. M.C.G. acknowledges a fellowship for postdoctoral training (20381/PD/17) funded by the Consejería de Empleo, Universidades y Empresa de la CARM, through the Fundación Séneca de la Región de Murcia.
dc.relation.publisherversionhttps://www.mdpi.com/2079-6374/11/1/18
dc.rightsAttribution 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectGold nanoparticles
dc.subjectGold nanoparticles
dc.subjectFumonsin B1
dc.subjectasymmetric flow field-flow fractionation
dc.subjectAptamers
dc.subjectUV/VIS spectroscopy
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleAptamer-Target-gold nanoparticle conjugates for the quantification of fumonisin B1.
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublicationes
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relation.isAuthorOfPublicationb9a16197-ec5b-45bf-a4a5-e0a4499b7d58
relation.isAuthorOfPublication.latestForDiscoveryba32f760-d55c-40a7-9452-62dfd47cbeaf
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