Publication: Radioautographic studies on radiosulfate incorporation in the digestive organs of mice
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Date
1999
Authors
Nagata, T. ; Morita, T. ; Kawahara, I.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The sulfate uptake and accumulation in
mouse digestive organs were studied by light microscopic
radioautography. Two litters of normal ddY mice
30 days after birth, each consisting of 3 animals, were
studied. One litter of animals were sacrificed 30 min
after the intra eritoneal injections with phosphate
buffered NaZ3gO4, and the other litter animals were
sacrificed 12 hr after the injections. Then several
digestive organs, the parotid gland, the submandibular
gland, the sublingual gland, antrum and fundus of the
stomach, the duodenum, the jejunum, the ileum, the
caecum, the ascending colon and the descending colon
were taken out. The tissues were fixed, dehydrated,
embedded in epoxy resin, sectioned, picked up onto
glass slides, coated with radioautographic emulsion by a
dipping method. After the exposure, they were
developed, stained with toluidine blue and analyzed by
light microscopy.
As the results, many silver grains were observed on
serous cells of the salivary glands, mucosa and submucosa
of the stomach, villous cells and crypt cells of
the small intestines and whole mucosa of the large
intestines at 30 min after the injection. Then at 12 hr
after the injection silver grains were observed on mucous
cells of the salivary glands, some of the stomach glands,
and mucigen granules of goblet cells in the small
intestines and the large intestines. The numbers of silver
grains observed in respective organs at 30 min were less
than those at 12 hr.
From these results, it is concluded that glycoprotein synthesis was demonstrated in several digestive organs
by radiosulfate incorporation. In the salivary glands the
silver grains were more observed in serous cells at 30
min, while in mucous cells more at 12 hr than 30 min
after the injection. In other organs the silver grains were
more at 30 min than at 12 hr. These results show the
time difference of glycoprotein synthesis in respective
organs.
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