Biocatalytic hydrolysis of di-urethane model compounds in ionic liquid reaction media

Salas, Rebeca; Villa Aroca, Rocío; Cano, Sergio; Garcia Verdugo, Eduardo; Lozano Rodríguez, Pedro; Nieto Cerón, Susana

Publication:
Biocatalytic hydrolysis of di-urethane model compounds in ionic liquid reaction media

dc.contributor.author	Salas, Rebeca
dc.contributor.author	Villa Aroca, Rocío
dc.contributor.author	Cano, Sergio
dc.contributor.author	Garcia Verdugo, Eduardo
dc.contributor.author	Villa Aroca, Rocío
dc.contributor.author	Lozano Rodríguez, Pedro
dc.contributor.author	Nieto Cerón, Susana
dc.contributor.department	Bioquímica y Biología Molecular B e Inmunología
dc.date.accessioned	2025-01-09T16:45:19Z
dc.date.available	2025-01-09T16:45:19Z
dc.date.issued	2024-03-15
dc.description	© 2024 The Author(s). This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/ This document is the Published Manuscript version of a Published Work that appeared in final form in Catalysis Today. To access the final edited and published work see https://doi.org/10.1016/j.cattod.2024.114516
dc.description.abstract	The suitability of different enzymes to carry out the hydrolysis of two-different toluene-based urethane model compounds (i.e. bis(2-methoxyethyl) (4-methyl-1,3-phenylene)dicarbamate, and bis(2-methoxyethyl) (2-methyl-1,3-phenylene)dicarbamate) has been demonstrated for the first time by taking advantage of ionic liquid (IL) technologies. Toluene-based urethane compounds were prepared from usual substrates in polyurethane industrial synthesis. Afterwards, their carbamate groups were target of a biocatalytic hydrolysis by means of different commercial hydrolases (i.e. lipase, urease and proteases) in either water, hydrophilic organic solvents (i.e. ethylene glycol or 1,2-dimethyl-1,3-dioxolane-4-methanol, (solketal)), or hydrophobic ILs (e.g. [C4mim][NTf2], etc.) as reaction media. Because of the insolubility of these compounds in water, most of the enzymes were unable to catalyse the hydrolysis of the di-urethane substrates in pure water, being clearly improved (up to 31.6 mU/mg for the urease case) in solketal:water (90:10, v/v) reaction media. When hydrophobic ILs were added into this reaction medium, the urease activity increased by more than twice (74.1 mU/mg). The most promising results for the hydrolysis of these urethane compounds were obtained by combining lipase and urease biocatalysts in a IL:solketal:H2O (70:25:5, v/v/v) reaction medium. These results demonstrate a possible biocatalytic approach for the hydrolytic depolymerization of polyurethane foam wastes.	es
dc.format	application/msword	es
dc.format.extent	7	es
dc.identifier.citation	Catalysis Today, 2024, Vol. 430 : 114516
dc.identifier.issn	Print: 0920-5861
dc.identifier.issn	Electronic: 1873-4308
dc.identifier.uri	http://hdl.handle.net/10201/148180
dc.language	eng	es
dc.publisher	Elsevier
dc.relation	This work was partially supported by MICINN-FEDER-AEI 10.13039/501100011033 (PID2021- 124695OB-C21/C22, and PDC2022-133313-C21/C22), MICINN –European Union Next Generation EU-PRTR (TED2021-129626B-C21/C22), and SENECA (21884/PI/22) for financial support.	es
dc.relation.publisherversion	https://www.sciencedirect.com/science/article/pii/S0920586124000105?via%3Dihub
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Atribución 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	*
dc.subject	Urethane hydrolysis	es
dc.subject	Urease
dc.subject	Lipase
dc.subject	Ionic liquid
dc.subject	Polyurethane wastes
dc.title	Biocatalytic hydrolysis of di-urethane model compounds in ionic liquid reaction media	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
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