Publication:
Circ_0000467 regulates proliferation, migration, invasion, and apoptosis in gastric cancer by targeting the miR-622/ROCK2 axis

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Tan-38-185-197-2023.pdf(13.82 MB)
Date
2023
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Authors
Tan, Shengquan ; Hu, Lingbo ; Lei, Rui ; Wang, Ruo ; Chen, Jiaquan
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/ 10.14670/HH-18-508
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info:eu-repo/semantics/article
Description
Abstract
Background. Gastric cancer (GC) ranks fourth as a cause of cancer-induced mortality worldwide. Recently, some studies have demonstrated that circular RNAs (circRNAs) play vital roles in human cancers, including GC. Methods. The expression levels of circ_0000467, microRNA-622 (miR-622), and Rho-associated coiledcoil-containing protein kinase2 (ROCK2) were determined by RT-qPCR assay. The protein expression was quantified by western blot assay. The interaction relationship between miR-622 and circ_0000467 or ROCK2 was confirmed by dual-luciferase reporter assay and RIP assay. The biological behaviors of GC cells including proliferation, apoptosis, migration, and invasion were determined by EdU assay, colony-forming assay, flow cytometry, and transwell assay. The effects of circ_0000467 silencing in vivo were assessed by a xenograft experiment in nude mice. Results. MiR-622 was downregulated and ROCK2 was upregulated in GC tissues and cells. Loss-offunction experiment revealed that overexpression of miR-622 decreased proliferation, migration, and invasion while it increased apoptosis in GC cells. Furthermore, ROCK2 was a functional target of miR622, and upregulation of ROCK2 abolished miR-622- induced effects on GC cells. What’s more, circ_0000467 was upregulated in GC, and inhibition of miR-622 reversed silencing of circ_0000467-caused effects on GC cells, suggesting that miR-622 was a target of circ_0000467. The suppression of circ_0000467 was able to slow the tumor growth in vivo. Conclusion. Mechanistically, circ_0000467 functioned as an oncogenic regulator in GC by specifically binding to miR-622 to upregulate ROCK2, which might be novel diagnostic markers for GC.
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Gastric cancer , circ_0000467 , miR-622 , ROCK2
Citation
Histology and Histopathology Vol. 38, nº2 (2023)
URI
http://hdl.handle.net/10201/129325
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Histology and histopathology Vol.38, nº2 (2023)
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