Publication:
Breastmilk polyamines modify gut gene expression in children at three months of age

Simple item page
dc.contributor.authorSánchez-Campillo, María
dc.contributor.authorGázquez García, Antonio
dc.contributor.authorPastor-Fajardo, María T.
dc.contributor.authorLópez-Andreo, María J.
dc.contributor.authorPérez-Andrés, Adrián
dc.contributor.authorMartínez Gracia, Carmen
dc.contributor.authorVioque, Jesús
dc.contributor.authorPérez Fernández, Virginia
dc.contributor.authorGarcía-Marcos Álvarez, Luis Vicente
dc.contributor.authorLarqué Daza, Elvira
dc.contributor.authorNela Study Group
dc.contributor.departmentTecnología de Alimentos, Nutrición y Bromatología
dc.contributor.otherFacultad de Veterinaria
dc.date.accessioned2026-01-22T18:49:33Z
dc.date.available2026-01-22T18:49:33Z
dc.date.copyright© 2025 The Authors
dc.date.issued2025-01-22
dc.description.abstractBackground & aims: Breastmilk is one of the main sources of exogenous polyamines for newborns and contains higher polyamine content than infant formulas. Polyamines are involved in gut maturation and immune system regulation in animals, although the underlying mechanisms are not fully understood. We quantified polyamines in human mature breastmilk and evaluate their effects on intestinal gene expression in babies at three months of age. Methods: Polyamines were quantified in mature milk of 195 women from the prospective Mediterranean NELA birth cohort (Murcia, Spain) at 3 months postpartum. Maternal dietary intake was assessed by food frequency questionnaires. Intestinal gene expression was analysed in babies receiving breastmilk, with high (>p50, n = 25) or low (<p50, n = 27) polyamine content, at 3 m of age, by microarray using a non-invasive method on gut exfoliated cells. Results: Spermidine and spermine were the most prevalent polyamines in breastmilk at 3 months postpartum, while levels of putrescine were lower. Maternal dietary intake of polyamines was not associated with polyamine concentration in breastmilk and there were no differences in the polyamine content between allergic and non-allergic mothers. Microarray analyses of exfoliated gut cells revealed that cell localization and immune system were the most significant biological processes affected by high vs low polyamines in breastmilk. There were 15 differentially expressed genes, 3 up-regulated and 12 down-regulated, in high compared to low polyamine groups. Among the up-regulated genes were tumour necrosis factor alpha-induced protein 6 (TNFAIP6) and interleukin 8; while other immune system-related genes, such as integral membrane protein 2C, lymphocyte antigen 6 complex, transmembrane protein 179B were down-regulated. Conclusions: Mature breastmilk presents spermidine and spermine as the most prevalent polyamines. Babies receiving milk with higher polyamine levels showed differences in the expression of genes associated with cell localization and immune system processes.
dc.formatapplication/pdf
dc.format.extent8
dc.identifier.citationClinical Nutrition 46 (2025) 80-87
dc.identifier.doihttps://doi.org/10.1016/j.clnu.2025.01.025
dc.identifier.eissn1532-1983
dc.identifier.issn0261-5614
dc.identifier.urihttp://hdl.handle.net/10201/191209
dc.languageeng
dc.publisherElsevier
dc.relationThis study was supported by grants from Institute of Health Carlos III (ISCIII), Spanish Ministry of Science, Innovation and Universities, and Fondos FEDER (grant numbers CP14/00046, PIE15/00051, PI16/00422, PI19/00863); ARADyAL Network (RD160006), Maternal and Child Health and Development Research Network (RICORS-SAMID, ISCIII RD21/0012), and Robles Chillida Foundation (R-1411/2019).
dc.relation.publisherversionhttps://www.clinicalnutritionjournal.com/article/S0261-5614(25)00026-3/fulltext
dc.rightsAttribution 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.odsObjetivo 3: Salud
dc.titleBreastmilk polyamines modify gut gene expression in children at three months of age
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublicationes
relation.isAuthorOfPublicationf894cb37-0ef8-4d53-89da-aa3b7f469c51
relation.isAuthorOfPublicationef169b38-3022-40ba-a2b2-131665b0d0d9
relation.isAuthorOfPublication35fa7756-a110-4e5a-b00d-e332beeff08f
relation.isAuthorOfPublicationb37b759a-7ec5-43f8-8547-b35602f94cdb
relation.isAuthorOfPublication0f4b679f-bfec-4a3f-8065-bafb202353ab
relation.isAuthorOfPublication.latestForDiscoveryf894cb37-0ef8-4d53-89da-aa3b7f469c51
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Breast milk polyamines.pdf
Size:
520.8 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.37 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Artículos