Publication: Comparative cytospectrophotometry of Wright-stained erythroid cells of vertebrates
Authors
Shigeyoshi Kamo ; Hisashi Takeuchi ; Masa-oki Yamada
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Visible spectra of erythroid cells stained with
Wright's method were studied microphotometrically.
Most mature cells of vertebrates have the main peak at
520 nm (A 520) and accessory peaks at 410 nm and 600 nm
(A410 and A600), respectively. To compare the
absorption at 520nm against 410nm and 600 nm, the
relative absorption spectra were determined and effectively
shown as percentage ratios (RAs) to the main peak of
A520. The A520 shifted to the shorter side of the
wavelengths in birds and lower vertebrates. When the RA
values of 410 and 600 nm were compared, it was observed
that RA 410 of immature cells was less than that of mature
cells (15%), and that immature cells of the lower RA 410
type were present in peripheral blood, as well as in bone
marrow of vertebrates. Thus, photometric analysis of
erythroid cells at RA 410 and RA 600 against A 520 seems
to provide useful parameters for comparative studies
on vertebrate erythroid cells. Six spectral types can be
observed in vertebrate erythroid cells by these parameters
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.