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New in-depth analytical approach of the porcine seminal plasma proteome reveals potential fertility biomarkers

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dc.contributor.authorPérez Patiño, Cristina
dc.contributor.authorParrilla Riera, Inmaculada
dc.contributor.authorBarranco Cascales, Isabel
dc.contributor.authorVergara Barberán, María
dc.contributor.authorSimó Alfonso, Ernesto F.
dc.contributor.authorHerrero Martínez, José M.
dc.contributor.authorRodríguez Martínez, Heriberto
dc.contributor.authorMartínez García, Emilio
dc.contributor.authorRoca Aleu, Jorge
dc.contributor.departmentMedicina y Cirugía Animal
dc.date.accessioned2025-10-16T12:40:43Z
dc.date.available2025-10-16T12:40:43Z
dc.date.copyright© 2018 American Chemical Society
dc.date.issued2018-02-07
dc.description.abstractA complete characterization of the proteome of seminal plasma (SP) is an essential step to understand how SP influences sperm function and fertility after artificial insemination (AI). The purpose of this study was to identify which among characterized proteins in boar SP were differently expressed among AI boars with significantly different fertility outcomes. A total of 872 SP proteins, 390 of them belonging specifically to Sus Scrofa taxonomy, were identified (Experiment 1) by using a novel proteomic approach that combined size exclusion chromatography and solid-phase extraction as prefractionation steps prior to Nano LC-ESI-MS/MS analysis. The SP proteomes of 26 boars showing significant differences in farrowing rate (n = 13) and litter size (n = 13) after the AI of 10 526 sows were further analyzed (Experiment 2). A total of 679 SP proteins were then quantified by the SWATH approach, where the penalized linear regression LASSO revealed differentially expressed SP proteins for farrowing rate (FURIN, AKR1B1, UBA1, PIN1, SPAM1, BLMH, SMPDL3A, KRT17, KRT10, TTC23, and AGT) and litter size (PN-1, THBS1, DSC1, and CAT). This study extended our knowledge of the SP proteome and revealed some SP proteins as potential biomarkers of fertility in AI boars.
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dc.identifier.citationJournal of Proteome Research, 2018, Vol. 17, N. 3, pp. 1065-1076
dc.identifier.doihttps://doi.org/10.1021/acs.jproteome.7b00728
dc.identifier.eissn1535-3907
dc.identifier.issn1535-3893
dc.identifier.urihttp://hdl.handle.net/10201/167069
dc.languageeng
dc.publisherAmerican Chemical Society
dc.relationThis experimental study was supported by MINECO and FEDER (AGL2015-69738-R and CTQ2014-52765-R) Madrid (Spain), Seneca Foundation (19892/GERM/15) Murcia (Spain), PROMETEO/2016/145 (Generalitat Valenciana, Spain), The Swedish Research Council (VR, 521-2011-6353), the Swedish Research Council Formas (221-2011-512), and the Research Council in Southeast Sweden (FORSS, 378091/312971), Sweden. C.P.-P. and M.V.-B. were financially supported by the Seneca Foundation (Murcia, Spain) and MINECO (Madrid, Spain), respectively. We are grateful to AIM Iberica (Topigs Norsvin Iberica) for supplying the boar ejaculates.
dc.relation.publisherversionhttps://pubs.acs.org/doi/10.1021/acs.jproteome.7b00728
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectFertility
dc.subjectPig
dc.subjectProteome
dc.subjectSeminal plasma
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleNew in-depth analytical approach of the porcine seminal plasma proteome reveals potential fertility biomarkers
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublicationes
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