Publication: Immunohistochemistry of new type I alveolar epithelial cell markers of the rat
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Date
1996
Authors
Kasper, M. ; Takeya, M. ; Takahashi, K. ; Grossmann, H. ; Schuh, D. ; Müller, M.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The presence of pan-cadherin and the MEP-1
antigen in normal and diseased rat lung was established
by employing immunoperoxidase and double label
fluorescence techniques. The binding of a mouse
monoclonal antibody (MEP-1) reacting specifically with
type 1 pneumocytes was assessed on paraffin sections of
normal specimens as well as those with pulmonary
fibrosis induced by bleomycin or radiation treatment. In
injured alveolar epithelium, a diminished type 1 cell and
a foca1 type 11 cell immunoreactivity was found.
Electron microscopy of immunogold-labelled lung tissue
confirmed the type 1 cell specificity of MEP-l. In
severely injured pulmonary parenchyma MEP-Inegative
areas occurred, which were also negative with
the type 11 pneumocyte marker Maclura pomifera lectin.
Similarly, a polyclonal pan-cadherin antibody uniformly
decorated luminal surfaces of alveoli except the type 11
pneumocytes. Furthermore, pleural mesothelial cells,
bronchiolar epithelial cells, endothelial cells of large
blood vessels and alveolar macrophage surfaces
exhibited pan-cadherin immunoreactivity. After injury, a
remarkable loss of pan-cadherin immunoreactivity in the
MEP-1-positive type 1 epithelial cells was detectable.
These findings suggest that characterization of normal
alveolar epithelial cells and monitoring of the epithelial
remodelling in pulmonary pathohistology are
sufficiently described by the antibodies MEP-1 anti pancadherin.
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